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Merck
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Fontos dokumentumok

MR-121

Sigma-Aldrich

EmbryoMax® KSOM Mouse Embryo Media

(1X), Liquid, with 1/2 Amino Acids & Phenol Red

Szinonimák:

K+ Simplex Optimised Medium (KSOM), KSOM Media

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

50 ML
20 100,00 Ft

20 100,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben
Összes fotó(1)

Méret kiválasztása

Nézet módosítása
50 ML
20 100,00 Ft

About This Item

UNSPSC kód:
12352207
eCl@ss:
32160801
NACRES:
NA.75

20 100,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben

Minőségi szint

300

Forma

liquid

gyártó/kereskedő neve

Specialty Media
EmbryoMax®

technika/technikák

cell culture | embryo: suitable
cell culture | stem cell: suitable

bemenet

sample type: mouse embryo(s)

Alkalmazás

EmbryoMax® KSOM Mouse Embryo Media has been used in the collection of mice embryos.[1][2]

Tárolás és stabilitás

Can be stored at -20°C upto the expiration date on the bottle.
Once thawed it should be used within 2 weeks.

Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.

Jogi információk

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Chao-Lien Liu et al.
The Journal of biological chemistry, 282(2), 1109-1118 (2006-11-17)
l(2)dtl (lethal (2) denticleless), is an embryonic lethal homozygous mutation initially identified in Drosophila melanogaster that produces embryos that lack ventral denticle belts. In addition to nucleotide sequence, bioinformatic analysis has revealed a conservation of critical functional motifs among the
Christiana Kyvelidou et al.
Journal of structural biology, 176(3), 379-386 (2011-10-04)
Embryo patterning is subject to intense investigation. So far only large, microscopically obvious structures like polar body, cleavage furrow, pro-nucleus shape can be evaluated in the intact embryo. Using non-linear microscopic techniques, the present work describes new methodologies to evaluate
A potential use of embryonic stem cell medium for the in vitro culture of preimplantation embryos.
Gelber K. et al.
Journal of Assisted Reproduction and Genetics null
Marina Gertsenstein et al.
PloS one, 5(6), e11260-e11260 (2010-06-29)
Genetically modified mouse strains derived from embryonic stem (ES) cells have become essential tools for functional genomics and biomedical research. Large scale mutagenesis projects are producing libraries of mutant C57BL/6 (B6) ES cells to enable the functional annotation of every
Hiroyuki Hirai et al.
Stem cells (Dayton, Ohio), 29(9), 1349-1361 (2011-07-07)
Induced pluripotent stem cells (iPSCs) can be created by reprogramming differentiated cells through introduction of defined genes, most commonly Oct4, Sox2, Klf4, and c-Myc (OSKM). However, this process is slow and extremely inefficient. Here, we demonstrate radical acceleration of iPSC
View All Related Papers

Cikkek

Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications

Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Embriókultúra médiumok

Egér embrió táptalajok és embrió validált reagensek transzgenikus egér embriók tenyésztéséhez

Questions

1–2 of 2 Questions  

  1. HI, DOES IT HAVE ANY LACTATE IN THE MEDIUM? IF SO, WHAT CONCENTRATION?

    1 answer

    1. This product does contain Lactate. Please see the full product formulation below. Quantities are in mg/L.

      Helpful?

  2. Is the KSOM (MR-121-D) sufficient for embryo culture to the blastocyst stage on its own? In the literature, papers mention using "KSOM supplemented with amino acids." I observed that MR-121-D indicates it has "half amino acids." Essentially, I am trying to understand if individuals are adding extra amino acids to this media, or if the 1/2 amino acids already included are what they are referring to when they say "supplemented with amino acids."

    1 answer

    1. KSOM (MR-121-D) is indeed sufficient for embryo culture up to the blastocyst stage. It is a 1X liquid solution with 1/2 amino acids and Phenol Red. When scientists reference KSOM with 1/2 amino acids, that is the intended composition, and they do not add the remaining 1/2 amino acids.

      For more detailed information and historical context, the article "A brief history of the development of the KSOM family of media" provides insights into the development and usage of KSOM. According to documented literature and the mentioned article, KSOM has been shown to support a high yield of mouse blastocysts capable of developing into fetuses after transfer to surrogate mothers. The medium was notably improved when supplemented with 19 natural amino acids, with the added amino acids present at half the concentration described by Eagle. Dose response studies indicated little difference in the response between full strength and half strength concentrations of the amino acids.

      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790120/

      Helpful?

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