MAB1864
Anti-Tubulin Antibody, clone YL1/2
clone YL1/2, Chemicon®, from rat
Szinonimák:
Anti-B-ALPHA-1, Anti-LIS3, Anti-TUBA3
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rat
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
YL1/2, monoclonal
faj reaktivitás
mouse, human, rat
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
izotípus
IgG2a
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... TUBA1A(7846)
Általános leírás
Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.
Egyediség
MAB1864 recognizes the α subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post-translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.
Immunogen
Epitope: The epitope recognizes a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
Yeast tubulin
Alkalmazás
Detect Tubulin using this Anti-Tubulin Antibody, clone YL1/2 validated for use in ELISA, IH, IP, RIA & WB.
Immunohistochemistry:
1:50-1:100 dilutoin from a previous lot was used on frozen sections.
ELISA:
1:100-1:1,000 dilution from a previousl lot was used.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Radioimmunoassy:
A previous lot of this antibody was used on Radioimmuoassy.
Optimal working dilutions must be determined by end user.
1:50-1:100 dilutoin from a previous lot was used on frozen sections.
ELISA:
1:100-1:1,000 dilution from a previousl lot was used.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Radioimmunoassy:
A previous lot of this antibody was used on Radioimmuoassy.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Minőség
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.
Cél megnevezése
55 kDa
Kapcsolódás
Replaces: 04-1117
Fizikai forma
Format: Purified
Protein A purified
Purified rat monoclonal IgG2a in buffer containing phosphate buffered saline (pH 7.4) with 0.09% sodium azide.
Tárolás és stabilitás
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analízis megjegyzés
Control
All tissue
All tissue
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline.
Williams, LS; Ganguly, S; Loiseau, P; Ng, BF; Palacios, IM
Development null
Integrin alpha 6 beta 4 promotes migration, invasion through Tiam1 upregulation, and subsequent Rac activation.
Cruz-Monserrate, Z; O'Connor, KL
Neoplasia null
Gem GTPase and tau: morphological changes induced by gem GTPase in cho cells are antagonized by tau.
Oyama, F; Kotliarova, S; Harada, A; Ito, M; Miyazaki, H; Ueyama, Y; Hirokawa, N; Nukina, N; Ihara, Y
The Journal of Biological Chemistry null
LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
Lee, S; Liu, HP; Lin, WY; Guo, H; Lu, B
The Journal of Neuroscience null
Peter T Ruane et al.
Scientific reports, 6, 27456-27456 (2016-06-09)
Microtubules and their associated proteins (MAPs) underpin the polarity of specialised cells. Adenomatous polyposis coli (APC) is one such MAP with a multifunctional agenda that requires precise intracellular localisations. Although APC has been found to associate with kinesin-2 subfamily members
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással