AB1603
Anti-Phosphoserine Antibody
Chemicon®, from rabbit
Szinonimák:
Anti-Phospho Serine Antibody, Phosphorylation Inhibitor
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(4)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
ELISA
IHC
IP
WB
IHC
IP
WB
technika/technikák:
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
84
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás (homológia által előrejelzett)
all
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
kiszállítva
wet ice
célzott transzláció utáni módosítás
phosphorylation (pSer)
Általános leírás
Cellular responses to a variety of extracellular signals occur through phosphorylation or dephosphorylation of intracellular proteins. Abnormal protein phosphorylation may be involved in the progression of numerous diseases, including many forms of cancer, immune system dysfunction and cardiovascular disease. Such changes in protein phosphorylation may be detected by immunocytochemical mapping of cells labeled with anti-phosphoserine or anti-phosphothreonine antibodies and can be correlated with cellular, electrophysiological or behavioral state changes.
Egyediség
Anti-phosphoserine is species independent.
Recognizes phosphoserine, peptidyl-phosphoserine, and serine-phosphorylated proteins. Does not cross react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross reactivity with free phosphothreonine. Readily reacts with known phosphoproteins such as phosvitin and alpha casein. Anti-phosphoserine is species independent in reactivity
Immunogén
Keyhole Limpet Hemocyanin(KLH)- conjugated phosphoserine conjugates.
Alkalmazás
Anti-Phosphoserine Antibody detects level of Phosphoserine & has been published & validated for use in ELISA, IH, IP & WB.
Immunoprecipitation (tissue extracts):
10-20 μg anti-pS/mg protein was used from a previous lot.
Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 μg/500 μL of extracts. Optimal concentrations must be determined experimentally.
ELISA (kinase assay):
A previous lot of this anitbody was used at a 1:250-1:500 dilution.
Immunohistochemistry:
A previous lot of this antibody was used at a 1:50 dilution.
As the antibody detects all phosphoserines, any phosphorylated serine protein or peptide can be used to block antibody staining.
Typically a 10 M excess of peptide is used in Tris based buffers.
Western Blot Analysis:
1:500 dilution of a previous lot
Optimal working dilutions must be determined by end user.
10-20 μg anti-pS/mg protein was used from a previous lot.
Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 μg/500 μL of extracts. Optimal concentrations must be determined experimentally.
ELISA (kinase assay):
A previous lot of this anitbody was used at a 1:250-1:500 dilution.
Immunohistochemistry:
A previous lot of this antibody was used at a 1:50 dilution.
As the antibody detects all phosphoserines, any phosphorylated serine protein or peptide can be used to block antibody staining.
Typically a 10 M excess of peptide is used in Tris based buffers.
Western Blot Analysis:
1:500 dilution of a previous lot
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Minőség
Routinely evaluated by Western Blot on NGF treated PC12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected serine phosphorylated proteins on 10 μg of NGF treated PC12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected serine phosphorylated proteins on 10 μg of NGF treated PC12 lysates.
Cél megnevezése
Dependent upon the molecular weight of the serine phosphorylated protein being detected.
Fizikai forma
Phosphoserine affinity chromatography
Purified rabbit serum in buffer containing 50% glycerol.
Tárolás és stabilitás
Stable for up to 6 months at -20°C in undiluted aliquots from date of receipt. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 uL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Analízis megjegyzés
Control
NIH 3T3 cells (+/- TPA), K562 cells, and EGF-stimulated A431 cells.
NIH 3T3 cells (+/- TPA), K562 cells, and EGF-stimulated A431 cells.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
A Crohn's disease-associated NOD2 mutation suppresses transcription of human IL10 by inhibiting activity of the nuclear ribonucleoprotein hnRNP-A1.
Noguchi, E; Homma, Y; Kang, X; Netea, MG; Ma, X
Nature Immunology null
A novel high throughput biochemical assay to evaluate the HuR protein-RNA complex formation.
D'Agostino, VG; Adami, V; Provenzani, A
Testing null
Pkd2+/- vascular smooth muscles develop exaggerated vasocontraction in response to phenylephrine stimulation.
Qian, Q; Hunter, LW; Du, H; Ren, Q; Han, Y; Sieck, GC
Journal of the American Society of Nephrology null
Liangzhen Jiang et al.
PloS one, 8(8), e72289-e72289 (2013-08-27)
Polo-like kinase 1 (Plk1) is a highly conserved Ser/Thr kinase in eukaryotes and plays a critical role in various aspects of the cell cycle. Plk1 exerts its multiple functions by phosphorylating its substrates. In this study, we found that Plk1
Bon-Hun Koo et al.
The Journal of biological chemistry, 287(27), 22643-22653 (2012-05-12)
Matrix metalloproteinase-2 (MMP-2) functions in diverse biological processes through the degradation of extracellular and non-extracellular matrix molecules. Because of its potential for tissue damage, there are several ways to regulate MMP-2 activity, including gene expression, compartmentalization, zymogen activation, and enzyme
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással