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Merck
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Fontos dokumentumok

05-1000X

Sigma-Aldrich

Anti-Phosphoserine Antibody, clone 4A4

clone 4A4, Upstate®, from mouse

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biológiai forrás

mouse

Minőségi szint

antitest forma

purified antibody

antitest terméktípus

primary antibodies

klón

4A4, monoclonal

faj reaktivitás

vertebrates

gyártó/kereskedő neve

Upstate®

technika/technikák

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

izotípus

IgG1

kiszállítva

wet ice

célzott transzláció utáni módosítás

phosphorylation (pSer)

Általános leírás

The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).

Egyediség

Serine-phosphorylated proteins from all species

Immunogen

Phosphoserine coupled to KLH

Alkalmazás

Anti-Phosphoserine Antibody, clone 4A4 is an antibody against Phosphoserine for use in WB, IF, FC, IH(P), ELISA.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Minőség

Routinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.

Cél megnevezése

Dependent upon the molecular weight of the serine phosphorylated protein being detected.

Fizikai forma

50 µL of PBS with 0.05% sodium azide with 30% glycerol.
Format: Purified
Protein G-Sepharose Chromatography

Tárolás és stabilitás

2 years at -20°C from date of shipment.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.

Jogi információk

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 2


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

SCF(Fbxw15) mediates histone acetyltransferase binding to origin recognition complex (HBO1) ubiquitin-proteasomal degradation to regulate cell proliferation.
Zou, C; Chen, Y; Smith, RM; Snavely, C; Li, J; Coon, TA; Chen, BB; Zhao, Y; Mallampalli, RK
The Journal of Biological Chemistry null
Avital Mendelson et al.
JCI insight, 4(16) (2019-08-23)
The complex process of platelet formation originates with the hematopoietic stem cell, which differentiates through the myeloid lineage, matures, and releases proplatelets into the BM sinusoids. How formed platelets maintain a low basal activation state in the circulation remains unknown.
Ying Yang et al.
Chemistry & biodiversity, 19(11), e202200333-e202200333 (2022-09-24)
N6-Methyladenosine (m6A), one of the post-transcriptional modifications of RNA, is important in hepatocellular carcinoma (HCC). However, the mechanism of its regulation remains elusive. We here show that exposure of HCC cells to sulfatide significantly reduced the total mRNA m6A modification.
Mario A Acuña et al.
The Journal of clinical investigation, 126(7), 2547-2560 (2016-06-09)
Diminished inhibitory neurotransmission in the superficial dorsal horn of the spinal cord is thought to contribute to chronic pain. In inflammatory pain, reductions in synaptic inhibition occur partially through prostaglandin E2- (PGE2-) and PKA-dependent phosphorylation of a specific subtype of
Vic Hart et al.
Journal of cancer research and clinical oncology, 147(10), 2893-2912 (2021-06-18)
In this study, two novel alternative splice variants of HER2, named HER2-PI9 and HER2-I12, were identified in breast cancer cell lines and breast tumour tissues. Whilst HER2-P19 arises from the inclusion of an 117 bp cassette-exon of intron 9 of HER2

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