420200

JC-1

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria.

• Szinonimák: JC-1, 5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide
• Tapasztalati képlet (Hill-képlet): C₂₅H₂₇Cl₄IN₄
• CAS-szám: 47729-63-5
• Molekulatömeg: 652.23
• UNSPSC kód: 12352202
• NACRES: NA.25

Tulajdonságok

• Minőségi szint: 100
• Teszt: ≥95% (HPLC)
• form: solid
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; protect from light
• szín: red-orange
• oldhatóság: DMSO: 5 mg/mL
• kiszállítva: ambient
• tárolási hőmérséklet: −20°C
• InChI: 1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1
• Nemzetközi kémiai azonosító kulcs: FYNNIUVBDKICAX-UHFFFAOYSA-M

Leírás

Általános leírás

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨ_m in cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of ΔΨ_m induced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨ_m in cells, tissues, and isolated mitochondria. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨ_m induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.

Biokémiai/fiziológiai hatások

Cell permeable: no

Primary Target
Used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria

Product does not compete with ATP.

Reversible: no

Kiszerelés

Packaged under inert gas

Figyelmeztetés

Toxicity: Irritant (B)

Feloldás

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.

Egyéb megjegyzések

Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
Gravance, C.G., et al. 2000. Theriogenology53, 1691.
Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995. Neuron 15, 961.
Reers, M., et al. 1995. Methods Enzymol. 260, 406.
Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable