P3375
Papaína from papaya latex
crude powder, 1.5-10 units/mg solid
Sinónimos:
Papinasa
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Número CAS:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
Specific activity:
1.5-10 units/mg solid
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Servicio técnico
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crude powder
Quality Level
specific activity
1.5-10 units/mg solid
mol wt
23.4 kDa
storage temp.
2-8°C
Application
Utilizada para producir fragmentos Fab de los anticuerpos. Se utiliza también para la disociación celular, ya que se ha demostrado que es más eficaz y menos lesiva con ciertos tejidos.
Papain from papaya latex has been used:
- in the enzyme cocktail to evaluate the efficacy of B complex vitamers liberation from nutritional yeast powder
- in the enzyme mixture for the digestion of bovine milk samples for the analysis of three vitamin B1 molecules
- as a biocatalyst for the hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate.
Biochem/physiol Actions
Una proteasa de cisteína que rompe los enlaces peptídicos de los aminoácidos básicos, la leucina o la glicina
pH óptimo 6,0-7,0
También hidroliza ésteres y amidas.
pH óptimo 6,0-7,0
También hidroliza ésteres y amidas.
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Analysis Note
Not standardized with lactose or other adulterants.
Other Notes
Véase más información sobre la papaína en www.sigma-aldrich.com/enzymeexplorer.
One unit will hydrolyze one micromole of N-alpha-benzoyl-l-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.
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Este artículo | |||
|---|---|---|---|
| specific activity 1.5-10 units/mg solid | specific activity ≥10 U/mg protein (~25 mg/ml) | specific activity - | specific activity >30,000 USP units/mg |
| form crude powder | form solution | form solid | form solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt 23.4 kDa | mol wt - | mol wt - | mol wt - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 100 |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Contenido relacionado
QC Methods
Comparison of different extraction methods for simultaneous determination of B complex vitamins in nutritional yeast using LC/MS-TOF and stable isotope dilution assay
Halvin K, et al.
Analytical and Bioanalytical Chemistry, 405(4), 1213-1222 (2013)
Carica papaya lipase: a novel biocatalyst for the enantioselective hydrolysis of (R, S)-naproxen 2, 2, 2-trifluoroethyl ester
Chen CC and Tsai SW
Enzyme and Microbial Technology, 36(1), 127-132 (2005)
Determination of the native forms of vitamin B1 in bovine milk using a fast and simplified UHPLC method
Schmidt A, et al.
Food Chemistry, 229, 452-457 (2017)
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| P3375-1KG | 04061834367789 |
| P3375-25G | 04061834367796 |
| P3375-100G | 04061834367772 |