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L4144

Lectina de Phaseolus vulgaris (frijol rojo)

Name: Lectina de <I>Phaseolus vulgaris</I> (frijol rojo)
Brand: SIGMA

Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

Sinónimos:

Aglutinina de Phaseolus vulgaris, PHA

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UNSPSC Code:

12352202

EC Number:

232-718-7

NACRES:

NA.32

Form:

lyophilized powder

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Servicio técnico

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Propiedades

Quality Segment

200

product line

BioReagent

form

lyophilized powder

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.), ≤1.0 μg per mL leucoagglutination activity, ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

suitability

leucocyte agglutination activity tested

storage temp.

2-8°C

Descripción

General description

Lectins are carbohydrate-binding proteins, which contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:

it has been used as a positive control to treat cells
to stimulate cell culture for the analysis of lymphocyte proliferation
to stimulate T cells

Biochem/physiol Actions

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It is associated with toxic allergens and hemagglutinins. Lectin exhibits anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.

La PHA consta de dos especies moleculares, la eritroaglutinina (PHA-E), que tiene poca actividad mitógena y elevada actividad de eritroaglutinina, y la leoucoaglutinina (PHA-L), que tiene elevada actividad mitógena y leoucoaglutinante, pero muy baja actividad eritroaglutininante
La PHA-E no es específica de grupo sanguíneo, pero la aglutinación puede ser inhibida por ciertos oligosacáridos. La PHA-P es la forma proteica de la PHA antes de la separación y la purificación de la eritroaglutinina y la leoucoaglutinina. La PHA-M es la forma mucoproteínica. Los conjugados se preparan a partir de las correspondientes lectinas purificadas.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (10⁷ per mL in saline) after 1 hr incubation at 25 °C.

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Este artículo	L1668	L8754	L2646
Sigma-Aldrich L4144 Lectin from Phaseolus vulgaris (red kidney bean)	Sigma-Aldrich L1668 Lectin from Phaseolus vulgaris (red kidney bean)	Sigma-Aldrich L8754 Lectin from Phaseolus vulgaris (red kidney bean)	Sigma-Aldrich L2646 Lectin from Phaseolus vulgaris (red kidney bean)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) -
form lyophilized powder	form lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)	form lyophilized powder	form lyophilized powder
suitability leucocyte agglutination activity tested	suitability -	suitability -	suitability -
impurities salt, free	impurities -	impurities salt, free	impurities salt, free
solubility DPBS: 5 mg/mL, slightly hazy, colorless	solubility PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)	solubility -	solubility -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number

0000479515

0000444443

SLCL9657

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Contenido relacionado

Data Sheet - L4144

Instructions

Número de artículo de comercio global

SKU	GTIN
L4144-5MG	04061833957295

Questions

Anonymous

2 years ago

What do the designations -P, -L, -M, and -E represent in the names of PHA products?

1 answer
1. Technical Support
  
  2 years ago
  
  Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:
  1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.
  2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.
  PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.
  
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