This product is filled by density, with a target fill volume of 0.5 ml for the 25mg and 2.0 ml for the 100mg package sizes.
A1602
Aminoglucosidasa from Aspergillus niger
ammonium sulfate suspension, ≥40 units/mg protein
Sinónimos:
1,4-α-D-Glucano glucohidrolasa, Exo-1,4-α-glucosidasa, Glucoamilasa
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥40 units/mg protein
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Servicio técnico
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ammonium sulfate suspension
Quality Segment
specific activity
≥40 units/mg protein
shipped in
wet ice
storage temp.
2-8°C
General description
Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.
Application
Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle [1].
Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.
Biochem/physiol Actions
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides [2].
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0
Other Notes
One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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Este artículo | |||
|---|---|---|---|
| specific activity ≥40 units/mg protein | specific activity ~70 U/mg | specific activity ≥260 U/mL | specific activity ~120 U/mg |
| form ammonium sulfate suspension | form powder | form aqueous solution | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
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Clase de almacenamiento
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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What volume of ammonium sulfate is the 25mg suspended in?
1 answer-
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How can a 1mg/mL solution of amyloglucosidase A1602 be prepared in acetate buffer?
1 answer-
The product A1602 is in an ammonium sulfate solution. Here's how you can work with this product:
- If the ammonium sulfate might interfere with the reaction(s):
1. Remove the enzyme from the refrigerator, ensuring that the ammonium sulfate suspensions are not frozen.
2. Gently invert the vial or bottle to produce a milky suspension. Avoid using harsh treatments like vortexing or sonicating, as they may denature some of the enzyme.
3. Use a pipette and sterile wide-bore pipette tip to remove a portion of the suspension.
4. Transfer the portion to a clean microcentrifuge or centrifuge tube.
5. Pellet the enzyme by centrifuging at approximately 10,000 – 15,000 x g for 10 minutes, if possible, using a refrigerated microcentrifuge set to a temperature between 2 °C and 8 °C.
6. Carefully remove as much clear supernatant as possible, retaining it; it is not necessary to remove absolutely all of the ammonium sulfate solution.
7. Dissolve the pellet by adding an appropriate amount of reaction buffer.
8. Assay the supernatant and the solution from the pellet for protein content and/or enzyme activity.- If the ammonium sulfate will not interfere with the reaction(s), follow the same steps as above.
It's important to note that for an ammonium sulfate suspension, most of the enzyme will be in solid form, and only negligible amounts of enzyme will likely be in the ammonium sulfate solution.
Alternatively, there are several Amyloglucosidase enzymes available in solid form (e.g., Product A7420 and A9228) that can be suspended in a solvent of your choice.
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