The enzymatic activity of this product is assessed at 60 °C with glucose as a substrate. External sources indicate higher temperatures, up to 75°C, may be optimal for more complex sugars.
10115
Aminoglucosidasa from Aspergillus niger
lyophilized, powder, ~70 U/mg
Sinónimos:
1,4-α-D-Glucano glucohidrolasa, Exo-1,4-α-glucosidasa, Glucoamilasa
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-877-2
MDL number:
Specific activity:
~70 U/mg
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powder
Quality Segment
quality
lyophilized
specific activity
~70 U/mg
mol wt
Mr ~97,000
greener alternative product characteristics
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Design for Energy Efficiency
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Greener Alternative Product
parameter
temperature at 50 °C (optimum temperature)
greener alternative category
, Enabling
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.
Amyloglucosidase is a disaccharide enzyme belonging to the α-glucosidase type. It is produced by several species of Aspergillus genus.
Amyloglucosidase is a disaccharide enzyme belonging to the α-glucosidase type. It is produced by several species of Aspergillus genus.
Application
Amyloglucosidase from Aspergillus niger has been used in glycogen assay and in acetate buffer for starch hydrolysis.[1] It can also be used for glycogen determination in whole yeast cells and synthesis of hetero-oligosaccharides by glucoamylase in reverse.
Other Notes
One unit corresponds to the amount of enzyme which liberates 1 μmole of glucose per minute at pH 4.8 and 60 °C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).
1 of 1
Este artículo | |||
|---|---|---|---|
| specific activity ~70 U/mg | specific activity ~120 U/mg | specific activity ≥40 units/mg protein | specific activity - |
| form powder | form powder | form ammonium sulfate suspension | form liquid |
| mol wt Mr ~97,000 | mol wt Mr ~97000 | mol wt - | mol wt - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| quality lyophilized | quality - | quality - | quality for use in Total Dietary Fiber Assay, TDF-100A |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the optimal temperature for this enzyme?
1 answer-
Helpful?
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Can you kindly indicate what the optimum pH is for this enzyme?
1 answer-
The activity of this enzyme is assessed at pH 4.8 with glucose as a substrate. External sources indicate a lower pH of approximately 4.0 may be optimal for a disaccharide, such as maltose, or a more complex sugar.
Helpful?
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What enzyme dilution buffer is recommended for this specific enzyme?
1 answer-
For assay purposes, this product is prepared in water acidified to pH 4.8 at a concentration of 5 u/mL. This material may also be prepared in 10 mM sodium acetate buffer, pH 4.8.
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