Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

93363

Sigma-Aldrich

Clorhidrato de Trizma®

BioUltra, for molecular biology, ≥99.0% (AT)

Sinónimos:

Clorhidrato de trometano, TRIS / HCI, TRIS hydrochloride, Tris(hidroximetil)-aminometano hydrochloride

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Fórmula lineal:
NH2C(CH2OH)3 · HCl
Número de CAS:
1185-53-1
Peso molecular:
157.60
Beilstein:
3675235
Número CE:
214-684-5
Número MDL:
MFCD00012590
Código UNSPSC:
12161700
ID de la sustancia en PubChem:
57653541
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grado

for molecular biology

Nivel de calidad

200

Línea del producto

BioUltra

Ensayo

≥99.0% (AT)

Formulario

powder or crystals

impurezas

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

residuo de ign.

≤0.2% (as SO4)

pérdida

≤0.2% loss on drying, 110 °C

pH

2.5-4.0 (25 °C, 4 M in H2O)

intervalo de pH útil

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubilidad

H2O: 4 M at 20 °C, clear, colorless

trazas de anión

sulfate (SO42-): ≤50 mg/kg

trazas de catión

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

4 M in H2O

Absorción UV

λ: 260 nm Amax: 0.12
λ: 280 nm Amax: 0.08

cadena SMILES

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

Clave InChI

QKNYBSVHEMOAJP-UHFFFAOYSA-N

¿Está buscando productos similares? Visita Guía de comparación de productos

Categorías relacionadas

Aplicación

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.

Otras notas

Los valores de pH de todas las disoluciones tampón son dependientes de la temperatura y la concentración. En el caso de los tampones Tris, el pH aumenta alrededor de 0,03 unidades por cada °C de disminución de la temperatura, y disminuye 0,03-0,05 unidades al ser diluido por 10. Para aplicaciones precisas, debe utilizarse un peachímetro cuidadosamente calibrado con un electrodo de vidrio/calomelano combinados.
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Component of extraction buffer e.g. phenol extraction of DNA or RNA[1]; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE[2].

Información legal

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
BCCM7499
BCCM1820
BCCM5867
BCCK3734
BCCH8205

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Santiago Vilanova et al.
Plant methods, 16, 110-110 (2020-08-15)
The use of sequencing and genotyping platforms has undergone dramatic improvements, enabling the generation of a wealth of genomic information. Despite this progress, the availability of high-quality genomic DNA (gDNA) in sufficient concentrations is often a main limitation, especially for
G G Nahas et al.
Drugs, 55(2), 191-224 (1998-03-20)
THAM (trometamol; tris-hydroxymethyl aminomethane) is a biologically inert amino alcohol of low toxicity, which buffers carbon dioxide and acids in vitro and in vivo. At 37 degrees C, the pK (the pH at which the weak conjugate acid or base
D Sirieix et al.
American journal of respiratory and critical care medicine, 155(3), 957-963 (1997-03-01)
Metabolic acidosis induces a decrease in the developed force of cardiac muscle by affecting every step of the excitation--contraction coupling pathway. Due to transient worsening in intracellular acidosis, the value of administering sodium bicarbonate therapeutically during acute acidosis has been
Characterization of in vitro translation products.
R Jagus
Methods in enzymology, 152, 296-304 (1987-01-01)
Large- and small-scale phenol extractions.
D M Wallace
Methods in enzymology, 152, 33-41 (1987-01-01)
Ver todos los artículos relacionados

Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

Reviews

No rating value

Active Filters

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico