Phosphate-buffered saline is a salt solution containing sodium phosphate and sodium chloride. In some formulations, it also contains potassium chloride and potassium phosphate. For example, products P4417 and P2272 contain both potassium chloride and sodium chloride.
P4474
Solution tampon phosphate
liquid, pH 7.4, sterile-filtered, suitable for cell culture
Synonyme(s) :
PBS
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About This Item
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Nom du produit
Solution tampon phosphate, pH 7.4, liquid, sterile-filtered, suitable for cell culture
Stérilité
sterile-filtered
Niveau de qualité
Forme
liquid
Technique(s)
cell culture | mammalian: suitable
Impuretés
Endotoxin, tested
pH
7.4
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Description générale
Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Why does this PBS not contain potassium chloride?
1 answer-
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Is it DNAs free?
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This product is intended for cell culture use and is not assayed for the presence of endonucleases. The specifications can be found at: https://www.sigmaaldrich.com/specification-sheet/SIGMA/P4474
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What is the conductivity of this solution?
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Unfortunately, the conductivity is not tested for this product as this is not a specification.
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What is the concentration (in mM) of this solution?
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This product is a multicomponent item. The concentration and molarity of each component is as follows:
Potassium phosphate monobasic - 0.144 g/L or 1.058mM
Sodium chloride - 9.0 g/L or 154mM
Sodium phosphate dibasic dihydrate - 0.528 g/L or 2.97mMHelpful?
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What is the composition of P4474 Phosphate buffered saline pH 7.4, liquid, sterile-filtered, suitable for cell culture ?
1 answer-
Product P4474 contains the following:
- 0.144 g/L Potassium phosphate monobasic
- 9.0 g/L Sodium chloride
- 0.528 g/L Sodium phosphate dibasic dihydrateHelpful?
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