If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
P4417
Solution tampon phosphate
tablet, pH 7.2-7.6 (1 tablet/200 mL)
Synonyme(s) :
PBS
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A propos de cet article
Nom du produit
Solution tampon phosphate, tablet
form
tablet
Quality Segment
storage condition
dry at room temperature
color
white
pH
7.2-7.6 (1 tablet/200 mL), 7.4
solubility
water: 1 tablet/200 mL, water: soluble
density
1.02 g/cm3 at 20—25 °C, 1.03 g/cm3 at 20—25 °C
application(s)
advanced drug delivery
diagnostic assay manufacturing
histology
life science and biopharma
General description
Au-delà de la biologie cellulaire, le PBS fait preuve de polyvalence, par exemple avec la création de tampons ELISA sur mesure pour optimiser les interactions anticorps-antigène. Il constitue également un tampon de migration reconnu pour la chromatographie d'affinité avec protéine A/G, facilitant la purification des anticorps en s'appuyant sur leur fixation à un antigène spécifique. En hématologie et en biochimie, le PBS a un rôle déterminant car il assure un lavage efficace des cellules et des tissus. En résumé, le PBS se démarque comme un outil indispensable tant dans la recherche en biologie qu'en biochimie, garantissant isotonicité, pouvoir tampon, simplicité et stabilité. Ses différentes applications en biologie cellulaire, dans la préparation de réactifs, la purification de protéines et divers tests biologiques montrent à quel point il fait progresser les connaissances scientifiques et notre compréhension.
Application
- production d'hydrocolloïdes à nanoparticules de platine (NP-Pt)
- lavage d'échantillons de sang et de platines de microscope avant microscopie électronique à balayage (MEB)
- préparation d'une solution d'agglutinine de germe de blé (WGA) marquée par une lectine fluorescente
- dilution de l'anticorps de capture lors d'un test ELISA (Enzyme Linked Immunosorbent Assay)
- mise en suspension de culots cellulaires lors d'une centrifugation
- techniques de dosage immunologique
Features and Benefits
- Produit de grande qualité convenant à de nombreuses applications de recherche.
- Peut être ajouté aux tampons de biologie et de biochimie.
- Forte solubilité dans l'eau.
Preparation Note
Other Notes
1 of 1
Cet article | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| application(s) advanced drug delivery | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) advanced drug delivery |
| form tablet | form liquid | form powder | form powder |
| solubility water: 1 tablet/200 mL, water: soluble | solubility - | solubility water: soluble | solubility water: soluble |
| density 1.03 g/cm3 at 20—25 °C, 1.02 g/cm3 at 20—25 °C | density - | density - | density 1.19 g/cm3 at 20—25 °C, 1.22 g/cm3 at 20—25 °C |
| storage condition dry at room temperature | storage condition - | storage condition - | storage condition dry at room temperature |
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Classe de stockage
13 - Non Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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How can I determine the shelf life / expiration / retest date of this product?
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After reconstitution of the tab, at which condition should I autoclave the PBS?
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The standard parameters of 121°C for 15 minutes at 15 lbs of pressure would be sufficient to sterilize a Phosphate Buffered Saline (PBS) solution.
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How much should one tablet weigh for it to yield 200ml 1xPBS? Asking just to make sure we won't use chipped tablets.
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Each tablet of this product should weigh between 1814.5 and 2005.5 milligrams.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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If I want to make the same buffer molarity as the product P4474-1L, how many tablets should I dissolve in 1L of DI water?
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P4417 and P4474 have different components and initial concentrations. One P4417 tablet dissolved in 200 mL of deionized water yields a 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride at pH 7.4, 25 °C. P4474 contains 1.058 mM potassium phosphate monobasic, 154 mM sodium chloride, and 2.97 mM sodium phosphate dibasic dihydrate. Due to these differences, it is not feasible to directly match the molarity of P4474 by dissolving P4417 tablets.
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Going by instructions, 1 tab in 200 mL water gives a 1x PBS sol'n. The recipe I use for 1x PBS has the same salt concs. Sorry if this is a silly question, but can I make up a 3x PBS solution simply by dissolving 3 tablets in 200 mL of water?
1 answer-
These tablets have not been tested for solubility or dissolution time at a 3X concentration. The end user would have to determine solubility experimentally. Note that 10X prepared solutions are available and may easily be diluted to the desired concentration. Please see the link below to review several of these product options:
https://www.sigmaaldrich.com/search/pbs-10x?focus=products&page=1&perpage=30&sort=relevance&term=PBS%2010X&type=productHelpful?
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Hi! If with this product I want to do a 10x solution, do i dilute it in 2000 mL or first in 200mL and then do the dilution? Thanks!
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Dissolve 1 tablet in 200 ml of water to obtain: 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4 At 25 °C. These instructions are for a 1 X PBS preparation and this product is only intended for this specific concentration. If a 10X concentrate is needed, there are options in the catalog, such as the following products : 79383, P7059, 6506-OP, and 6505-OP.
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Is it possible to obtain the salt components for product 6501-200TAB? Additionally, can you provide the composition details for other PBS tablets or powder products?
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The compositions are as follows:
1. 6501-200TAB: This PBS contains 137mM NaCl, 2.7 mM KCl, and 10mM Na phosphate.
2. P3813 - powder - Packed in Pouch or PAK: Each pouch contains 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride, totaling 9.55 g per packet. This is a standard PBS formulation.
3. P4417- Tablet: When dissolved in 200 mL of deionized water, one tablet yields 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride, at pH 7.4 and 25 °C. This product contains the same formulation as P3813 on a per liter basis (excluding tableting fillers): 8 g/L Sodium Chloride, 0.2 g/L Potassium Phosphate, monobasic, 1.15 g/L Sodium Phosphate, dibasic, and 0.2 g/L Potassium Chloride. This mixture should be diluted to one liter of water, resulting in a pH of 7.4, which can be adjusted using phosphoric acid or sodium hydroxide. The concentrations are 10 mM phosphate buffer, 2.7 mM potassium chloride, and 137 mM. These PBS tablets do not contain BSA.
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What is the osmolality of P4417 made to 1X PBS at pH 7.4?
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This PBS once diluted in 200 mL distilled water will account for:
2.7 mM potassium chloride = 5.4 mosmoles / L
137 mM sodium chloride = 274 mosmoles / LThe exact osmolality is not determined.
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Is this PBS calcium-free?
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The formulation of this product is considered proprietary. However, calcium is not included in the composition.
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