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M5524

Murashige and Skoog Basal Salt Mixture (MS)

powder, suitable for plant cell culture

Synonyme(s) :

MS Basal Salts, MS0 Basal Salts, MSO Basal Salts

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A propos de cet article

NACRES:
NA.72
UNSPSC Code:
12352207
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form

powder

Quality Segment

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

General description

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter).

Application

Murashige and Skoog Basal Salt Mixture (MS) has been used to support the growth and regeneration of transformed tobacco cells. It has also been used as a growth medium for germination of Arabidopsis thaliana (Col-0) seeds.

Preparation Note

Formulated to contain 4.3 grams of powder per liter of medium.
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
With the macro- and micronutrients as described by Murashige and Skoog (1962).

Media Formulation

Other Notes

Storage
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.

Store dry medium in a desiccator at 0-5 °C.

Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.

Media Preparation

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Cet article
M2909M6899M0529
form

powder

form

powder

form

powder

form

liquid

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

shipped in

ambient

shipped in

-

shipped in

-

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

400


pictograms

Flame over circleExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Classe de stockage

5.1B - Oxidizing hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Consulter la Bibliothèque de documents



Chien-Yu Huang et al.
Plant physiology, 174(4), 2248-2260 (2017-06-15)
Plant cytosolic lipid droplets (LDs) are covered with a layer of phospholipids and oleosin and were extensively studied before those in mammals and yeast. Oleosin has short amphipathic N- and C-terminal peptides flanking a conserved 72-residue hydrophobic hairpin, which penetrates
Tohru Ariizumi et al.
The Plant cell, 20(9), 2447-2459 (2008-10-02)
This article presents evidence that DELLA repression of gibberellin (GA) signaling is relieved both by proteolysis-dependent and -independent pathways in Arabidopsis thaliana. DELLA proteins are negative regulators of GA responses, including seed germination, stem elongation, and fertility. GA stimulates GA
Pyae Phyo et al.
Plant physiology, 175(4), 1593-1607 (2017-11-01)
At early stages of Arabidopsis (



Numéro d'article de commerce international

RéférenceGTIN
M5524-10L04061838259769
M5524-50L04061838259776
M5524-1L04061835568864

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