Heat inactivation is required based on the specific application or protocol. It is typically necessary for hybridoma cultures or immunological studies that require the inactivation of complement proteins. However, heat inactivation is not required for all applications.
F2442
Sérum de veau fœtal
USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
Synonyme(s) :
FBS, FCS, sérum, sérums
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| Conditionnement | Référence | Disponibilité | Prix |
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A propos de cet article
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
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Laissez-nous vous aiderbiological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
Le sérum de veau fœtal (SVF ou FBS en anglais) est un mélange complexe qui contient des molécules de bas et haut poids moléculaires et qui présente des fonctions optimales pour la stimulation et l'inhibition de la croissance. Le FBS contient des molécules comme des facteurs de croissance, des protéines, des vitamines, des oligoéléments, des hormones, etc. Ces molécules sont importantes pour la croissance et le maintien des cellules. C'est un supplément pour milieux de culture cellulaire d'usage courant. Le FBS est obtenu à partir de sang de veau. Il est dépourvu de fibrine et de facteurs de coagulation. Son inactivation par la chaleur inhibe le système du complément. Le FBS dérivé de sang coagulé est le supplément non défini le plus utilisé dans la culture des cellules eucaryotes et notamment des cellules de mammifères.[1] Il est généralement utilisé avec les milieux classiques mis au point dans les années 1950 et 1960. Ces derniers comprennent de nombreux milieux très prisés, comme le milieu essentiel minimum (MEM)[2], le milieu d'Eagle modifié par Dulbecco (DME ou DMEM), le milieu de Dulbecco modifié par Iscove (IMDM)[3], le milieu M199[4] et le milieu RPMI 1640 (Roswell Park Memorial Institute).[2]
Application
Le sérum de veau fœtal entre également dans la composition des milieux et liquides suivants :
Biochem/physiol Actions
Le FBS reste un supplément pour milieux très prisé, car il assure de multiples fonctions en culture cellulaire. Il apporte des nutriments, des facteurs de croissance et d'adhésion et protège les cellules des dommages oxydatifs et de l'apoptose par des mécanismes qui sont difficiles à reproduire avec les milieux sans sérum.
Analysis Note
- A fait l'objet de tests pour rechercher la présence de bactéries, de virus et de mycoplasmes
- Filtré trois fois sur membrane de 0,1 micron en conditions aseptiques
- Taux d′endotoxines et d′hémoglobine testés
Other Notes
Produit testé pour la présence de bactéries, de virus, de mycoplasmes et d'endotoxines et pour la teneur en hémoglobine. Produit filtré trois fois sur membrane de 0,1 µm en conditions aseptiques.
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Cet article | |||
|---|---|---|---|
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility γ-irradiated, sterile-filtered |
| origin USA origin | origin USA origin | origin USA origin | origin non-USA origin |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin |
| composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤20 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤25 mg/dL |
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Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Contenu apparenté
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001
Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)
Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| F2442-100ML | 04061838338709 |
| F2442-1L | 04061837034589 |
| F2442-50ML | 04061838255396 |
| F2442-24X500ML | 04061838255372 |
| F2442-500ML | 04061838255389 |
| F2442-6X500ML | 04061838255402 |
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is heat inactivation necessary?
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How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)
1 answer-
Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.
Here's a protocol for heat inactivation:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.Helpful?
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is this Heat Inactivate FBS?
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This product has not been heat inactivated.
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can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?
1 answer-
This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317Helpful?
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Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks
1 answer-
Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/Helpful?
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What is the level of growth factors in Fetal Bovine Serum, Product F2442?
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Sigma Aldrich does not determine the levels of growth factors (such as TGF beta, VEGF, EGF, PDGF, or FGF) for serum with reduced levels of growth factors and plasma derived serum.
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Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?
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We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.
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What is the difference between Fetal Bovine Serum F2442 and 12103C?
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Product No. 12103C meets most of the specifications of F2442. Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.
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Does Sigma-Aldrich offer fetal bovine serum (FBS) that is antibiotic-free?
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FBS can be tested for the presence of antibiotics. Products F2442, F0926, and F6178, Fetal Bovine Serum are routinely tested for Tetracycline (by far the most commonly used antibiotic in animals) and the results are reported on the lot specific Certificate of Analysis.Per the USDA, Antibiotics may be given to prevent or treat disease in cattle. A "withdrawal" period is required from the time antibiotics are administered until it is legal to slaughter the animal. This is so residues can exit the animal's system. FSIS randomly samples cattle at slaughter and tests for residues. Data from this Monitoring Plan have shown a very low percentage of residue violations.The USDA does random sampling of all cattle submitted for slaughter in USDA inspected facilities for antibiotics. The mandatory withdrawal period is 24 hours. Based on withdrawal periods required by the USDA, animal testing at slaughter and finished product testing, our serum is antibiotic free.
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Are all Fetal Bovine Serum (FBS) products heat inactivated?
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Not all Fetal Bovine Serum products are heat inactivated. Product numbers F4135 and 12306C are heat-inactivated FBS.
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