The FBS should be stored at the recommended temperature of -20°C. Storage at -80°C has not been tested, but there are no anticipated issues. Frost Free Freezers should be avoided for storage.
F4135
Sérum de veau fœtal
Fetal Bovine Serum
USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma
Synonyme(s) :
FBS HI, FCS HI, Sérum HI, sérums HI, FBS, FCS, sérum, sérums
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About This Item
Source biologique:
bovine fetus
Origine:
USA origin
Stérilité:
sterile-filtered
Conditions d'expédition:
dry ice
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
bovine fetus
Niveau de qualité
Stérilité
sterile-filtered
Gamme de produits
FBS Classic
Composition
Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL
Origine
USA origin
Technique(s)
cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable
Impuretés
≤10 EU/mL endotoxin
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Description générale
Le sérum de veau fœtal (SVF, ou FBS en anglais) reste un supplément pour milieux très prisé, car il assure de multiples fonctions en culture cellulaire. Le FBS apporte des nutriments, des facteurs de croissance et d'adhésion et favorise la prolifération cellulaire.[1] En outre, il protège les cellules des dommages oxydatifs et de l'apoptose.[2] Le FBS inhibe la formation d'espèces réactives de l'oxygène, ces dernières n'étant pas éliminées dans les milieux sans sérum.[3]
Application
Le sérum de veau fœtal originaire des États-Unis est utilisé dans une multitude d'applications de culture cellulaire. Il apporte aux cellules en culture de nombreux facteurs de croissance et de survie non définis.
Le sérum de veau fœtal a été utilisé :
Le sérum de veau fœtal a été utilisé :
- dans le milieu d'Eagle modifié par Dulbecco (DME ou DMEM)[4], le milieu essentiel minimum d'Eagle (EMEM)[5], le milieu RPMI 1640 (Roswell Park Memorial Institute)[6], la modification α du milieu d'Eagle (αMEM)[7] et le milieu L-15 de Leibovitz[8]
- dans un milieu de blocage pour le traitement de coupes de tronc cérébral[9]
- pour améliorer l'adhésion d'explants cutanés[10]
Notes préparatoires
Préparé par chauffage à 56 °C pendant 30 minutes.
This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. L'inactivation à la chaleur inactive également d'autres inhibiteurs indéterminés de la croissance des cellules en culture.
Remarque sur l'analyse
- A fait l'objet de tests pour rechercher la présence de bactéries, de virus et de mycoplasmes
- Filtré trois fois sur membrane de 0,1 micron en conditions aseptiques
- Taux d′endotoxines et d′hémoglobine testés
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
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Certificats d'analyse (COA)
Lot/Batch Number
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Articles
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Contenu apparenté
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
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Is it possible to store the FBS at a temperature of -80°C, which is lower than the recommended storage temperature of -20°C?
1 answer-
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Recommended storage is listed as -20C; is it safe/ok to store at -80C?
1 answer-
The recommended long-term storage temperature for FBS is -20C. For the short-term, such as for shipping, packing on dry ice at -40C is recommended. It is not recommended to store the FBS at temperatures lower than -40C. Please see the guide on freezing and thawing FBS and other sera below:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/storing-and-thawing-serumHelpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why is serum Heat-Inactivated (HI)?
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Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
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Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
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Does Sigma-Aldrich offer delipidated bovine serum?
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At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss
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