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03978

Methylene Blue solution

concentrate according to Ehrlich, concentrated, aqueous solution

Synonyme(s) :

Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue

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A propos de cet article

Formule empirique (notation de Hill) :
C16H18ClN3S
Numéro CAS:
Poids moléculaire :
319.85
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
3641570
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Quality Segment

form

liquid

quality

concentrated, aqueous solution

shelf life

~3 yr

concentration

1.5% (1.5 g/ 100 mL)

density

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

General description

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.
Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.
Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

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Cet article
50484319112457250
suitability

suitable for microscopy

suitability

-

suitability

-

suitability

-

concentration

1.5% (1.5 g/ 100 mL)

concentration

0.5% in H2O (0.5 g/100 mL)

concentration

0.05 wt. % in H2O

concentration

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

microbiology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

form

liquid

form

-

form

liquid

form

solid

Quality Level

100

Quality Level

-

Quality Level

200

Quality Level

100

shelf life

~3 yr

shelf life

-

shelf life

-

shelf life

-


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wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Classe de stockage

12 - Non Combustible Liquids



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Questions

  1. Concerning the stated concentration of the product 03978 (Methylene blue solution, 1.5%), what is the uncertainty and lot-to-lot variability?

    1 answer
    1. Product 03978 is not a GMP quality product. No testing is performed during Quality Control testing that measures lot to lot variability. The testing includes tests for Appearance, Thin Layer Chromatography, Density, Wavelength 1 at 656-666nm, Extinction Coefficient for Wavelength 1 and the solvent used to measure the wavelength and extinction coefficient. The information about a 1.5% concentration of dye is from the production documents used to manufacture the dye solution. The actual dye concentration is not measured.

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