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46944

Fluorescein isothiocyanate–dextran

average mol wt 4,000, (FITC:Glucose = 1:250)

Synonym(s):

FITC-Dextran 4, FITC–Dextran

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About This Item

CAS Number:
60842-46-8
UNSPSC Code:
12352201
NACRES:
NA.32
MDL number:
MFCD00131092
Pricing and availability is not currently available.
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Quality Level

100

form

powder

mol wt

average mol wt 4,000

composition

(FITC:Glucose = 1:250)

concentration

90-100 % (w/w) (TRITC DEXTRAN)

extent of labeling

~0.004 mol/mol FITC glucose

color

orange to dark orange, yellow to dark yellow

solubility

H2O: 50 mg/mL, slightly hazy, orange

storage temp.

2-8°C

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination
Fluorescein isothiocyanate–dextran (FITC-Dextran 4) is use as a fluorescent probe to study cell permeability. FITC-Dextran 4 is used with to study permeability of epithelial cell such as intestinal and renal cells in vivo.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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This Item
4694646945FD4
Sigma-Aldrich

Sigma-Aldrich

46944

Fluorescein isothiocyanate–dextran

Sigma-Aldrich

Sigma-Aldrich

46946

Fluorescein isothiocyanate–dextran

Sigma-Aldrich

Sigma-Aldrich

46945

Fluorescein isothiocyanate–dextran

Fluorescein isothiocyanate–dextran average mol wt 3,000-5,000

Sigma-Aldrich

FD4

Fluorescein isothiocyanate–dextran

solubility

H2O: 50 mg/mL, slightly hazy, orange

solubility

H2O: 25 mg/mL, slightly hazy, very deep yellow-orange

solubility

H2O: 50 mg/mL, hazy, orange to very deep orange

solubility

H2O: 25 mg/mL

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

powder

form

solid

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

orange to dark orange, yellow to dark yellow

color

-

color

light orange to very dark orange, light yellow to very dark yellow

color

light yellow to dark orange

extent of labeling

~0.004 mol/mol FITC glucose

extent of labeling

~0.004 mol/mol FITC glucose

extent of labeling

~0.004 mol/mol FITC glucose

extent of labeling

0.001-0.020 mol FITC per mol glucose

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pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP2957
BCCN6537
BCCM8453
BCCM4014
BCCK8819

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Development of a novel self-microemulsifying drug delivery system for reducing HIV protease inhibitor-induced intestinal epithelial barrier dysfunction.
Lei B, Zha W, Wang Y, Wen C, et al.
Molecular Pharmacology, 7, 844-853 (2010)
Adam J Moeser et al.
Research in veterinary science, 93(2), 975-982 (2012-01-31)
The objective of this study was to investigate intestinal function and morphology associated with peri-weaning failure to thrive syndrome (PFTS) in swine. Jejunum and distal ileum from control and pigs exhibiting PFTS was harvested at weaning, 4 and 11 days
Rebekah E Dumm et al.
Nature communications, 10(1), 779-779 (2019-02-17)
Influenza B virus (IBV) is an acute, respiratory RNA virus that has been assumed to induce the eventual death of all infected cells. We and others have shown however, that infection with apparently cytopathic viruses does not necessarily lead to
View All Related Papers

Global Trade Item Number

SKUGTIN
46944-500MG-F04061832358871
46944-100MG-F04061832358864

Questions

1–7 of 7 Questions  

  1. Can FITC-dextran 4KDa be view through florescence microscope to assess the gut permeability of Balb/c mice?

    1 answer

    1. Unfortunately, there is no internal data related to using this product to assess intestinal permeability. However, FITC-Dextran 4 was previously cited in this context. Kindly review the following citations:

      Tagesson C, Sjödahl R, Thorén B. Passage of molecules through the wall of the gastrointestinal tract. I. A simple experimental model. Scand J Gastroenterol. 1978;13(5):519-24. doi: 10.3109/00365527809181758. PMID: 705246.

      Ahmad R, Rah B, Bastola D, Dhawan P, Singh AB. Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching. Sci Rep. 2017 Jul 11;7(1):5125. doi: 10.1038/s41598-017-04989-8. PMID: 28698546; PMCID: PMC5505957.

      Voetmann LM, Rolin B, Kirk RK, Pyke C, Hansen AK. The intestinal permeability marker FITC-dextran 4kDa should be dosed according to lean body mass in obese mice. Nutr Diabetes. 2023 Jan 5;13(1):1. doi: 10.1038/s41387-022-00230-2. PMID: 36604407; PMCID: PMC9816099.

      Helpful?

  2. We would like to use this Product 46944-500MG for in vivo barrier integrity assays. Is there any data on the in vivo stability in lamina propria immune cells? We do not want the FITC Dextran Assay to interfere with Flow Cytometry Analysis.

    1 answer

    1. This product is not tested for the specific application, and data on in vivo stability in lamina propria immune cells is not available. A literature search is recommended for information regarding this application.

      Helpful?

  3. Are there any fluorescent hydrophobic substitutes of similar size to FITC dextran (4,000 amu) available, considering regular dextran lacks fluorescence and is hydrophilic?

    1 answer

    1. There is only one dextran conjugated with a hydrophobic fluorescent dye. The TRITC dextran, T4037, has an average dextran weight of 4,400 amu and TRITC is slightly more hydrophobic than FITC.

      Helpful?

  4. At what excitation and emission wavelengths should Product No. 46944, Fluorescein isothiocyanate-dextran, be read?

    1 answer

    1. The excitation maximum for FITC-dextran is 490 nm, while the emission maximum is 520 nm.

      Helpful?

  5. What is the recommended storage method for stock solutions of item 46944, Fluorescein isothiocyanate-dextran?

    1 answer

    1. Once Fluorescein isothiocyanate-dextran (item 46944) is in solution, it is recommended to store it at -20ºC and utilize it within 3 months to maintain its potency. To prevent multiple freeze/thaw cycles, it is advisable to aliquot the solution.

      Helpful?

  6. After reconstitution, how can we sterilise the dextran solution?

    1 answer

    1. The FITC-Dextran solution can be sterilized by filtration.

      Helpful?

  7. What is the stability of FITC-dextran in vivo?

    1 answer

    1. These products are not tested on animals. Reports suggest a 24-hour in vivo stability.

      Helpful?

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