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Merck

PLED35

Sigma-Aldrich

GenElute Endotoxin-free Plasmid Midiprep Kit

greener alternative

sufficient for 35 purifications

Sinónimos:

GenElute Endotoxin-free Plasmid Kits, endo free midiprep plasmid isolation kit, endotoxin free plasmid extraction kit, endotoxin free plasmid isolation kit, endotoxin free plasmid purification kit, midiprep plasmid isolation kit, plasmid extraction kit, plasmid purification kit, Gen Elute

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

usage

sufficient for 35 purifications

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

greener alternative category

storage temp.

15-25°C

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General description

The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.

Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 μg of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The kit has been used for purifying plasmid DNA, which has been used for transfection.

Features and Benefits

  • High transfection efficiency and yield
  • Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
  • Fast and simple allowing up to 12 preps in less than 2 hours

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Herbst S
PLoS ONE, 6(5), e19105-e19105 (2011)
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Bondi ML
Journal of Drug Targeting, 15(4), 295-301 (2007)
Karyotypically abnormal human ESCs are sensitive to HDAC inhibitors and show altered regulation of genes linked to cancers and neurological diseases.
Lund RJ
Stem Cell Research, 11(3), 1022-1036 (2013)
Comparison of different signal peptides for protein secretion in nonlytic insect cell system.
Olczak M and Olczak T
Analytical Biochemistry, 359(1), 45-53 (2006)

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