A0919

Mouse IgG−Agarose

(Suspension in 0.5 M NaCl containing preservative.)

• Synonym(s): IgG-Agarose Beads
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00164808
• Conjugate: agarose conjugate
• Technique(s): immunoprecipitation (IP): suitable
• Application: IP
• Citations: 92

Properties

• conjugate: agarose conjugate
• Quality Segment: 200
• antibody form: purified immunoglobulin
• form: (Suspension in 0.5 M NaCl containing preservative.)
• extent of labeling: 1 mg per mL
• technique(s): immunoprecipitation (IP): suitable
• matrix: Cross-linked 4% beaded agarose
• matrix activation: cyanogen bromide
• matrix spacer: 1 atom
• storage temp.: 2-8°C

Description

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases[1]. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice[2].

Application

IgG-Agarose can be used as an immunoadsorbent. It can also be used to affinity purify antibodies, remove species specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgGAgarose (resin volume). Mouse IgG-Agarose has been used for several immunoprecipitation assays[3],[4].

Mouse IgGs conjugated to agarose beads were used in immunoprecipitation assays to pre-clear HEK 293 whole cell extracts. Beads were incubated with extracts for 1 hour rotating at 4°C.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Other Notes

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Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1