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A6284

Sigma-Aldrich

Human IgG−Agarose

suspension

Synonym(s):

IgG Agarose

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

agarose conjugate

Quality Level

form

suspension

extent of labeling

≥5 mg per mL

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

storage temp.

2-8°C

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Application

IgG-Agarose is an immuno-adsorbent that can be used to affinity purify antibodies, remove species-specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically, cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgG-Agarose (resin volume). However, the resin to antiserum ratio will vary with individual applications.
Tandem affnitiy purification pulldown was performed in yeast cultures using agarose-linked human IgG. Supernatants were mixed with 30μl of agarose beads overnight.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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