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P0425

Poly-Prep Slides

poly-L-lysine coated glass slides

Synonyme(s) :

Poly-L-Lysine-coated glass slides

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A propos de cet article

NACRES:
NA.47
UNSPSC Code:
41116124
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Quality Segment

200

shelf life

Expiry date on the label.

application(s)

hematology
histology

storage temp.

room temp

General description

Poly-Prep slides are made of glass and designed for cell adhesion. The slides are coated with Poly-L-Lysine, a non-specific attachment factor for cells useful in promoting cell adhesion on solid substrates. Poly-L-lysine enhances binding between the cell membrane and the cultured surface.

Application

Poly-Prep Slides are used:
  • To mount liver and lung cell sections and mounted on poly-prep slides.
  • To prove better cell adhesion in clinical bronchoalveolar lavage fluid samples.
  • To determine pAkt (alpha serine/threonine-protein kinase) expression by immunohistochemistry.
  • In immunofluorescence imaging of nuclear translocation of nuclear factor κB(NFκB) in A549 cells.
  • To study the phospholipase C-zeta activity of sperm.
  • To prepare coelomocytes smears, which were further subjected to antibody staining.
Overall, glass slides coated with poly-L-lysine for tissue section adhesion in routine histological uses. And, it is not recommended for cell culture or microarray applications.

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Cet article
Z721158CLS294875X25Z721107
Poly-Prep Slides poly-L-lysine coated glass slides

Sigma-Aldrich

P0425

Poly-Prep Slides

FAST slides for protein microarrays 16 pad, 6 x 6 mm, pk/10

Z721158

FAST slides for protein microarrays

Corning® microscope slides, frosted one side, one end L × W 75 mm × 25 mm

CLS294875X25

Corning® microscope slides, frosted one side, one end

FAST slides for protein microarrays 1 pad, barcoded, 20 x 51 mm, pk/20

Z721107

FAST slides for protein microarrays

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

application(s)

hematology
histology

application(s)

-

application(s)

-

application(s)

-

shelf life

Expiry date on the label.

shelf life

-

shelf life

-

shelf life

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

3-aminopropyltriethoxysilane, tissue section, tissue adhesive microscope slides, poly-l-lysine, histology

-

-

-

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000444899
0000444899
0000363312
0000319176
0000363312

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Clinical significance of Akt and HER2/neu overexpression in African-American and Latina women with breast cancer
Wu Y, et al.
Breast Cancer Research, 10(1), R3-R3 (2008)
Amhed M Vargas-Velazquez et al.
Genetics, 211(4), 1315-1330 (2019-02-01)
Genetic screens in the nematode Caenorhabditis elegans identified the EGF/Ras and Notch pathways as central for vulval precursor cell fate patterning. Schematically, the anchor cell secretes EGF, inducing the P6.p cell to a primary (1°) vulval fate; P6.p in turn
The effect of prolonged incubation and temperature on oocyte activator phospholipase C-zeta activity of sperm
Kervancioglu DE, et al.
Marmara medical journal, 629-629 (2017)
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Numéro d'article de commerce international

RéférenceGTIN
P0425-72EA04061835573776

Questions

1–6 of 6 Questions  

  1. Is it positively charged?

    1 answer

    1. The Poly Prep slides (P0425) are positively charged.

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  2. How many slides will be present?

    1 answer

    1. This product comes in a pack of 72 slides.

      Helpful?

  3. Would you recommend wiping the slides with ethanol before use? What alternatives can be used if not ethanol? I'm seeing some artifacts in microscopy which is not from the dyes or tissue.

    1 answer

    1. Assuming the Poly-Prep Slides are clean, it is doubtful that dipping the slides into ethanol or other solvents will decrease the artifact you are seeing. Poly-Prep slides have been noted to have blue fluorescence in the UV range. There are reference articles that mention blue fluorescence of L-Lysine in the UV range. The full PDF can be downloaded at the URL provided below. In house experience with the polymer matches that of the monomer.

      https://www.sciencedirect.com/science/article/pii/S1386142522008666

      Helpful?

  4. What's size of the slides?

    1 answer

    1. These slides are rectangular with frosting at one edge.
      The dimensions are given as 3 inches (~75mm) x 1 inch (~25mm) with a thickness of 1mm.
      The coated area is approx 18 to 20 mm from one edge.

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  5. How can the side of the slide that is poly-L-lysine coated be differentiated?

    1 answer

    1. The coating is visible as white. Using a lead pencil to identify which side of the surface has been coated is suggested, as the lead will mark the coated side but not the uncoated glass.

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  6. How many slides comes into the package?

    1 answer

    1. There are 72 slides in each package of this item.

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