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P0425

Sigma-Aldrich

Poly-Prep Slides

poly-L-lysine coated glass slides

Synonyme(s) :

Poly-L-Lysine-coated glass slides

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About This Item

Code UNSPSC :
41116124
Nomenclature NACRES :
NA.47
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Niveau de qualité

200

Durée de conservation

Expiry date on the label.

Application(s)

hematology
histology

Température de stockage

room temp

Description générale

Poly-Prep slides are made of glass and designed for cell adhesion. The slides are coated with Poly-L-Lysine, a non-specific attachment factor for cells useful in promoting cell adhesion on solid substrates. Poly-L-lysine enhances binding between the cell membrane and the cultured surface.

Application

Poly-Prep Slides are used:
  • To mount liver and lung cell sections and mounted on poly-prep slides.
  • To prove better cell adhesion in clinical bronchoalveolar lavage fluid samples.
  • To determine pAkt (alpha serine/threonine-protein kinase) expression by immunohistochemistry.
  • In immunofluorescence imaging of nuclear translocation of nuclear factor κB(NFκB) in A549 cells.
  • To study the phospholipase C-zeta activity of sperm.
  • To prepare coelomocytes smears, which were further subjected to antibody staining.
Overall, glass slides coated with poly-L-lysine for tissue section adhesion in routine histological uses. And, it is not recommended for cell culture or microarray applications.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000363312
0000319176
0000363312
SLCP5057
SLCR4237

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Tissue processing/embedding cassettes

The effect of prolonged incubation and temperature on oocyte activator phospholipase C-zeta activity of sperm
Kervancioglu DE, et al.
Marmara medical journal, 629-629 (2017)
Clinical significance of Akt and HER2/neu overexpression in African-American and Latina women with breast cancer
Wu Y, et al.
Breast Cancer Research, 10(1), R3-R3 (2008)
Amhed M Vargas-Velazquez et al.
Genetics, 211(4), 1315-1330 (2019-02-01)
Genetic screens in the nematode Caenorhabditis elegans identified the EGF/Ras and Notch pathways as central for vulval precursor cell fate patterning. Schematically, the anchor cell secretes EGF, inducing the P6.p cell to a primary (1°) vulval fate; P6.p in turn
M Nizzardo et al.
Acta neuropathologica, 139(5), 837-853 (2020-02-18)
In amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA), spinal and lower brainstem motor neurons degenerate, but some motor neuron subtypes are spared, including oculomotor neurons (OMNs). The mechanisms responsible for this selective degeneration are largely unknown, but the
Yu Zheng et al.
Nature communications, 8, 14344-14344 (2017-02-10)
Metastasis-competent circulating tumour cells (CTCs) experience oxidative stress in the bloodstream, but their survival mechanisms are not well defined. Here, comparing single-cell RNA-Seq profiles of CTCs from breast, prostate and lung cancers, we observe consistent induction of β-globin (HBB), but
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Questions

1–3 of 3 Questions  

  1. What's size of the slides?

    1 answer

    1. These slides are rectangular with frosting at one edge.
      The dimensions are given as 3 inches (~75mm) x 1 inch (~25mm) with a thickness of 1mm.
      The coated area is approx 18 to 20 mm from one edge.

      Helpful?

  2. How can the side of the slide that is poly-L-lysine coated be differentiated?

    1 answer

    1. The coating is visible as white. Using a lead pencil to identify which side of the surface has been coated is suggested, as the lead will mark the coated side but not the uncoated glass.

      Helpful?

  3. How many slides comes into the package?

    1 answer

    1. There are 72 slides in each package of this item.

      Helpful?

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