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M5519

Milieu de base de Murashige et Skoog

powder, suitable for plant cell culture

Synonyme(s) :

Milieu de base MS

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A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.72
MDL number:
MFCD00240976
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form

powder

Quality Segment

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

General description

Le milieu de Murashige et Skoog est un milieu de culture des tissus de plantes largement utilisé. Le milieu de base M&S contient des macronutriments qui incluent des taux élevés de nitrates et des additifs organiques comme de la gélose, des sucres, des vitamines et des régulateurs de croissance. Parmi les régulateurs de croissance importants ajoutés au milieu M&S, on compte l′auxine/morphogène IAA et la kinétine, une cytokinine et agent promoteur de la division cellulaire.

Application

Le milieu de Murashige et Skoog a été utilisé dans le milieu de culture des tissus de plantes pour l′étude de la transformation de plantes.[1][2]

Preparation Note

Comporte des macronutriments, des micronutriments et des vitamines comme décrit par Murashige et Skoog (1962).

Formulation du milieu
Le milieu de Murashige et Skoog peut être reconstitué à partir d′une poudre ou en combinant des produits constituants majeurs du milieu complet M&S, dont les mélanges de macronutriments et de vitamines. Le milieu de Murashige et Skoog (M5519) contient les micronutriments et vitamines de la formulation classique d′origine. Il peut être additionné de saccharose, de gélose, d′auxines (IAA) et de cytokinines (kinétine) pour former un milieu complet apte à la croissance des tissus de plantes.
Produit formulé pour contenir 4,4 grammes de poudre par litre de milieu.

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Cet article
M0404M7024M9274
Sigma-Aldrich

Sigma-Aldrich

M5519

Milieu de base de Murashige et Skoog

Sigma-Aldrich

Sigma-Aldrich

M0404

Milieu de base de Murashige et Skoog

Murashige and Skoog Shoot Multiplication Medium A powder, suitable for plant cell culture

Sigma-Aldrich

M7024

Murashige and Skoog Shoot Multiplication Medium A

Milieu de base de Murashige et Skoog suitable for plant cell culture, with sucrose and agar

Sigma-Aldrich

M9274

Milieu de base de Murashige et Skoog

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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pictograms

Flame over circleExclamation mark
GHS03,GHS07

signalword

Warning

hcodes

H272,H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Classe de stockage

5.1B - Oxidizing hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–10 of 11 Questions  

  1. How do I prepare this medium?

    1 answer

    1. The preparation instructions for this product are as follows:
      1. Using a container twice the size of the desired final volume, measure out ~90% of the required final volume of tissue culture grade water (e.g., Catalog Number W3500). Example: 900 ml for a final volume of 1000 ml.
      2. While stirring, add the powdered product.
      3. Rinse the original container with a small volume of tissue culture grade water to remove traces of the powder. Add to the solution in step 2.
      4. Add desired supplements (e.g., sucrose, gelling agent, auxins, cytokinins).
      Add heat labile constituents after autoclaving.
      5. While stirring, adjust to the desired pH (e.g., 5.7±0.1) using KOH, NaOH, or HCl.
      6. Add additional tissue culture grade water to bring the medium to the final volume.
      7. If a gelling agent is used, heat the solution to clarity while stirring.
      8. Dispense the medium into culture vessels before or after autoclaving according to the application.
      Add heat labile constituents after autoclaving.
      9. Sterilize the medium in a validated autoclave at 1 kg/cm2 (15 psi). The medium should attain a temperature of 121 °C for at least 15 minutes.

      Please see the link below to review this and additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Helpful?

  2. What is the difference between Product M5519, Murashige and Skoog (MS) Basal Medium and M5524, Murashige and Skoog Basal Salt Mixture?

    1 answer

    1. Product M5524 is the Murashige and Skoog(M&S) Basal Salt Mixture, only. The M5519 products is M&S Basal Medium which also includes the vitamins myo-Inositol, Nicotinic acid and Pyridoxine HCl. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      Helpful?

  3. What is the formulation of Product M5519, Murashige and Skoog (MS) Basal Medium?

    1 answer

    1. Please see the link below to review the product datasheet, including the full formulation:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      To review additional Murashige and Skoog formulations, please see the link below:

      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog

      Helpful?

  4. Product M5519, Murashige and Skoog Basal Medium, is on back order. Can you suggest an alternate?

    1 answer

    1. Product M5524 Murashige and Skoog(M&S) Basal Salt Mixture, is an ideal alternative to M5519, M&S Basal Medium. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      Helpful?

  5. Can I autoclave Product M5519, Murashige and Skoog Basal Medium?

    1 answer

    1. Yes, this medium is autoclavable.

      Helpful?

  6. once the media is prepared, what conditions should it be stored in, and how long can it be stored?

    1 answer

    1. The prepared solution is quite stable but should be stored refrigerated. Monitor the salt solution for particulates or turbidity. After the addition of agar and subsequent sterilization, poured plates or vessels should also be stored aseptically in the refrigerator until use. The shelf life of the stored plates or vessels is most often limited by less stable compounds added to the medium. The end user would have to determine the stability of these components. While there is no firm shelf life on the prepared medium, contamination, and drying are the most common reasons for degradation. Monitor plates/vessels for contaminants or turbidity. An indication of excessive drying will be the gelled medium pulling away from the sides of the plate or vessel. The medium has lost moisture and may not be suitable for use. Moisture loss can be minimized by storing prepared plates/vessels in a sealed container or sealed bags. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Helpful?

  7. What is the percentage of sucrose in M5519?

    1 answer

    1. The M5519 does not contain sucrose. This component would have to be added prior to autoclaving. The typical amount is 30 g/L. See the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Should sucrose be desired, see product M9274, which is more of a complete formulation containing M&S salts, vitamins, sucrose, and agar. See the link below to review the formulations of the various Murashige & Skoog media:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog#Basal%20Salts%20Mixtures

      Helpful?

  8. What is the quantity and what is shelf life

    1 answer

    1. The SKU sizes indicated refer to the final volume of media that can be made upon reconstitution of the pre-weighted powder. This powdered media is formulated to contain 4.4 grams of powder per liter of medium. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services

      Helpful?

  9. How much agar is there in Product M5519, Murashige and Skoog Basal Medium?

    1 answer

    1. This product does not contain any agar.

      Helpful?

  10. Product M5519, Murashige and Skoog Basal Medium, has precipitates; will that affect the growth?

    1 answer

    1. Precipitates are known to occur, with time, in plant tissue culture media.  The precipitates have been analyzed.  They are composed of small pale yellow-white particles.  Analysis of precipitates indicated a predominance of iron, phosphate, and zinc.  The probable cause of the precipitates is the inevitable oxidation of ferrous ions to ferric ions and the presence of unchelated ferric ions.  When the solubility of ferric phosphate is exceeded precipitation occurs.  There are no reports of detrimental effects on growth and development in plant tissue culture due to the precipitates.

      Helpful?

1–10 of 11 Questions  

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