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A8592

Anticorps monoclonal ANTI-FLAG® M2-peroxydase (HRP) antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

ANTI-FLAG® M2-Peroxidase (HRP) antibody, Mouse monoclonal

Synonyme(s) :

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk

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A propos de cet article

NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
peroxidase conjugate
Clone:
M2, monoclonal
Application:
ELISA (i)
Citations:
1904
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biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GPX1(2876)

General description

L′anticorps monoclonal ANTI-FLAG M2-peroxydase est une IgG de souris conjuguée de manière covalente à de la peroxydase de raifort (HRP). L′anticorps se fixe aux protéines de fusion portant une étiquette FLAG et reconnaît l′épitope FLAG des peptides FLAG en position interne et sur les extrémités N-terminale, Met-N-terminale et C-terminale.

Application

Dilution suggérée pour l'immunocytochimie et l'analyse par western blotting : 1/100 à 1/1000.
Dilution suggérée pour l'analyse ELISA : 1/20 000.

Pour en savoir plus sur nos produits, rendez-vous sur notre portail des applications des produits FLAG®.
Pour la détection simple en une étape par analyse immunocytochimique, ELISA ou western blot. Particulièrement utile dans la détection des protéines de fusion avec étiquette FLAG exprimées chez un hôte murin, où les anticorps anti-souris peuvent entraîner une réactivité croisée.

Physical form

Solution dans une solution saline tamponnée au phosphate contenant 50 % de glycérol, ainsi qu′un conservateur et un stabilisant.

Preparation Note

Diluer la solution d′anticorps ANTI-FLAG M2-peroxydase dans une solution saline tamponnée au Tris (TBS) : Tris 0,05 M, pH 7,4, avec NaCl 0,15 M.

Other Notes

Lauréat du prix CiteAb 2024 récompensant les avancées des fournisseurs dans la recherche sur la maladie de Parkinson

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Cet article
A9469F3040SAB4301135
ANTI-FLAG® M2-Peroxidase (HRP) antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

A8592

ANTI-FLAG® M2-Peroxidase (HRP) antibody, Mouse monoclonal

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

A9469

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse

Monoclonal ANTI-FLAG® M1 antibody clone M1, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F3040

Monoclonal ANTI-FLAG® M1 antibody

Anti-FLAG Tag antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4301135

Anti-FLAG Tag antibody produced in rabbit

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

clone

M2, monoclonal

clone

M2, monoclonal

clone

M1, monoclonal

clone

polyclonal

biological source

mouse

biological source

mouse

biological source

(Bioreactor)

biological source

rabbit

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

technique(s)

indirect ELISA: 1:20,000

technique(s)

indirect ELISA: 1:20,000

technique(s)

western blot: 10 μg/mL

technique(s)

western blot: 1:1000-1:5000 (Cell Lysate)

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Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

Classe de stockage

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000554016
0000554018
0000554017
0000554017
0000554018

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Contenu apparenté

Numéro d'article de commerce international

RéférenceGTIN
A8592-5X1MG04061833396889
A8592-.2MG04061835511761
A8592-1MG04061833392409
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Questions

1–7 of 7 Questions  

  1. I am currently using your product A8592 HRP in my analytical method with QuantaBlu fluorescence substrate complex. Is it possible to use TMB substrate instead of QuantaBlu fluorescence substrate with the A8592 HRP conjugate?

    1 answer

    1. A8592 is an HRP-conjugated antibody. The product information sheet can be downloaded here:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/306/a8592dat-mk.pdf

      Although we do not routinely test 3,3′,5,5′-tetramethylbenzidine (TMB) in-house, it is a standard chromogenic substrate for horseradish peroxidase, so it is expected to work with the A8592 conjugate.

      QuantaBlu from ThermoFisher is a fluorogenic substrate; TMB is colorimetric.
      You should expect lower sensitivity with TMB compared with QuantaBlu, as fluorescence detection generally offers higher sensitivity.
      The TMB-HRP reaction produces a soluble end-product that is blue in color and can be read spectrophotometrically at 370 nm or 650 nm.
      The reaction may be stopped with acid, resulting in a yellow solution that is read at 450 nm.

      Published protocol for use of A8592 together with TMB include:

      https://pmc.ncbi.nlm.nih.gov/articles/PMC3709174/pdf/cpb-0026-0363.pdf
      https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.869825/full
      https://www.nature.com/articles/s41556-024-01448-1

      Helpful?

  2. What are the appropriate storage conditions for Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody?

    1 answer

    1. This product ships on wet ice and it is recommended to store the undiluted antibody at 0 to -20°C.  Once diluted, repeated freezing and thawing is not recommended.

      Helpful?

  3. Can product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, be used for immunohistochemistry?

    1 answer

    1. The product cannot be used on paraffin embedded tissue sections, but it does work on fixed culture cells (as indicated on the Product Information Sheet) and also may work on frozen sections.

      Helpful?

  4. How is Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, supplied?

    1 answer

    1. Product No. A8592, ANTI-FLAG M2-peroxidase is supplied as a solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 50% glycerol, plus stabilizer and preservative.  The conjugate protein concentration is approximately 1 mg/ml.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. I am getting a high background on my Western blots using Product No. A8592, Monoclonal Anti-FLAG® M2-Peroxidase (HRP) antibody.  What can I do to fix this?

    1 answer

    1. There is a troubleshooting guide provided at the end of the product information sheet.  Some suggestions include the following:  adjusting antibody titer, increase number of washes, increase stringency of washes by addition of 0.05% TWEEN 20 or 0.1% TRITON X-100 to the wash buffers, and/or increase the incubation and wash temperatures.

      Helpful?

  7. What concentration of Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, is recommended for my application?

    1 answer

    1. An antibody titer from 1:100 to 1:1000 is recommended for immunocytochemistry and Western blot.  Minimum antibody titer recommended for ELISA is 1:20,000.  Adjust antibody titer to maximize detection sensitivity and to minimize background.

      Helpful?

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