The stability of this material is solution has not been determined. However, aqueous solutions of 25 mg/mL or more of FITC-Dextran are expected to be stable for 1-2 weeks when stored refrigerated at 2-8°C and protected from light. For long-term storage, aliquot and store stock solutions at ?20°C, protected from light.
46945
Fluorescein isothiocyanate–dextran
average mol wt 70,000, (FITC:Glucose = 1:250)
Synonym(s):
FITC-Dextran 70, FITC–Dextran
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About This Item
Pricing and availability is not currently available.
biological source
synthetic
Quality Segment
conjugate
FITC conjugate
form
powder
mol wt
average mol wt 70,000
composition
(FITC:Glucose = 1:250)
concentration
90-100 % (w/w) (TRITC DEXTRAN)
extent of labeling
~0.004 mol/mol FITC glucose
color
light orange to very dark orange, light yellow to very dark yellow
solubility
H2O: 50 mg/mL, hazy, orange to very deep orange
fluorescence
λex 492 nm; λem 518 nm in ethanol
application(s)
cell analysis
storage temp.
2-8°C
Application
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
Fluorescein isothiocyanate–dextran (FITC-Dextran 70) is use as a fluorescent probe to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-Dextran 70 may be used to study the capacity, selectivity and efficiency of encapsulation and release by drug delivery vehicles.
Other Notes
Internalisation of FITC and FITC-dextran by suspension cultured plant cells; In the study of fluid pinocytosis in leukocytes[1]
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This Item | |||
|---|---|---|---|
| biological source synthetic | biological source synthetic | biological source synthetic | biological source synthetic |
| solubility H2O: 50 mg/mL, hazy, orange to very deep orange | solubility - | solubility - | solubility - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form powder | form powder or crystals | form powder or crystals | form powder or crystals |
| storage temp. 2-8°C | storage temp. - | storage temp. - | storage temp. - |
| color light orange to very dark orange, light yellow to very dark yellow | color - | color - | color - |
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signalword
Warning
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 46945-100MG-F | 04061832358895 |
| 46945-500MG-F | 04061832358901 |
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After reconstituting in water, what are the storage conditions?
1 answer-
Helpful?
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Is this product fixable?
1 answer-
Yes, paraformaldehyde can be used to fix cells. In normal circumstances, formaldehyde is a cross-linking fixative that is used to fix protein and DNA.
Looking at formaldehyde fixation, the following are applicable:
Formaldehyde cross-links with the amino group of the N-terminal amino acid residue and the side chains of 12:
Arginine
Cysteine
Histidine
Lysine
Depending on the peptide sequence, methylol groups, Schiff-bases, and methylene bridges were formPlease note that although the term paraformaldehyde is used, paraformaldehyde exists only as a solid. Once the bonds of the polymer are broken, solutions contain only formaldehyde. Any paraformaldehyde in the solution would simply be an undissolved precipitate at the bottom of the solution.
Helpful?
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