Enhancing biocompatibility of some cation selective electrodes using heparin modified bacterial cellulose.

Enhancing biocompatibility of some cation selective electrodes using heparin modified bacterial cellulose.

Carbohydrate polymers (2015-10-03)

Ibrahim H A Badr, R Abdel-Sattar, Sherif M A S Keshk

RESUMO

Bacterial cellulose (BC) and heparin-modified bacterial cellulose (HBC) were utilized to enhance the biocompatibility of highly thrombogenic PVC-based potassium and calcium membrane electrodes. Three types of membrane electrodes were prepared: (1) conventional PVC electrode (control), (2) PVC-based electrode sandwiched with bacterial cellulose membrane (BC-PVC), and (3) PVC-based electrode sandwiched with heparin-modified bacterial cellulose membrane (HBC-PVC). The potentiometric response characteristics of the modified potassium and calcium membrane electrodes (BC-PVC and HBC-PVC) were compared with those of the control PVC-based potassium and calcium selective electrode, respectively. Response characteristics of the modified membrane electrodes were comparable to the control PVC membrane electrode. The platelet adhesion investigations indicated that (BC) and (HBC) layers are less thrombogenic compared to PVC. Therefore, use of BC or HBC would enable the enhancement of the biocompatibility of PVC-based membrane electrodes for potassium and calcium while practically maintaining the overall electrochemical performance of the PVC sensing film.

MATERIAIS

Número do produto

Marca

Descrição do produto

T1503

Sigma-Aldrich

Trizma^® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

S9763

Sigma-Aldrich

Fosfato de sódio, ACS reagent, ≥99.0%

360589

Sigma-Aldrich

Tetra-hidrofurano, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%

G8270

Sigma-Aldrich

D-(+)-Glicose, ≥99.5% (GC)

T6066

Sigma-Aldrich

Trizma^® base, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

G7021

Sigma-Aldrich

D-(+)-Glicose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

71640

Sigma-Aldrich

Fosfato de sódio, puriss. p.a., ACS reagent, anhydrous, ≥99.0% (T)

D9434

Sigma-Aldrich

Dextrose, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications

27109

Sigma-Aldrich

Ácido cítrico, meets analytical specification of Ph. Eur., BP, USP, E330, anhydrous, 99.5-100.5% (based on anhydrous substance)

401757

Sigma-Aldrich

Tetra-hidrofurano, anhydrous, ≥99.9%, inhibitor-free

T6687

Sigma-Aldrich

Tromethamine, meets USP testing specifications

84822

Sigma-Aldrich

Bis(2-ethylhexyl) sebacate, ≥97.0% (GC)

G7528

Sigma-Aldrich

D-(+)-Glicose, ≥99.5% (GC), BioXtra

T1378

Sigma-Aldrich

Sigma 7-9^®, ≥99% (titration), crystalline

04276

Sigma-Aldrich

Fosfato de sódio, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E 339, anhydrous, 98-100.5% (calc. to the dried substance)

93362

Sigma-Aldrich

Trizma^® base, BioUltra, for molecular biology, ≥99.8% (T)

186562

Sigma-Aldrich

Tetra-hidrofurano, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%

93352

Sigma-Aldrich

Trizma^® base, ≥99.0% (T)

178810

Sigma-Aldrich

Tetra-hidrofurano, ReagentPlus^®, ≥99.0%, contains 250 ppm BHT as inhibitor

87368

Sigma-Aldrich

Tetra-hidrofurano, contains 250 ppm BHT as inhibitor, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.9%

115533

Sigma-Aldrich

CDI, reagent grade

S3264

Sigma-Aldrich

Fosfato de sódio, for molecular biology, ≥98.5% (titration)

S5136

Sigma-Aldrich

Fosfato de sódio, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%

94046

Sigma-Aldrich

Sodium phosphate dibasic solution, BioUltra, 0.5 M in H₂O

T6791

Sigma-Aldrich

Trizma^® base, BioXtra, pH 10.5-12.0 (1 M in H₂O), ≥99.9% (titration)

49139

Sigma-Aldrich

D-(+)-Glicose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)

21860

Sigma-Aldrich

CDI, ≥97.0% (T)

251275

Sigma-Aldrich

Ácido cítrico, ACS reagent, ≥99.5%

252859

Sigma-Aldrich

Tris(hidroximetil)-aminometano, ACS reagent, ≥99.8%

47249

Supelco

D-(+)-Glicose, analytical standard