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94046

Sigma-Aldrich

Sodium phosphate dibasic solution

BioUltra, 0.5 M in H2O

Sinônimo(s):

Disodium hydrogen phosphate solution, di-Sodium hydrogen phosphate solution

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About This Item

Fórmula linear:
Na2HPO4
Número CAS:
Peso molecular:
141.96
Número MDL:
Código UNSPSC:
12161700
eCl@ss:
38070211
ID de substância PubChem:
NACRES:
NA.25

linha de produto

BioUltra

forma

liquid

concentração

0.5 M in H2O

Impurezas

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

solubilidade

H2O: 0.5 M at 20 °C, clear, colorless

densidade

1.064 g/mL at 20 °C

traços de ânion

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

traços de cátion

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

0.5 M in H2O

Absorção UV

λ: 260 nm Amax: 0.019
λ: 280 nm Amax: 0.016

aplicação(ões)

diagnostic assay manufacturing

cadeia de caracteres SMILES

[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

chave InChI

BNIILDVGGAEEIG-UHFFFAOYSA-L

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Aplicação

Sodium phosphate dibasic solution has been used:
  • to supplement Dulbecco′s Modified Eagle Medium (DMEM) in calcifying medium to induce the calcification of vascular smooth muscle cells (VSMCs)
  • to prepare a stock solution of CaCl2 and MgCl2 to synthesize calcium phosphate (CPB) and magnesium phosphate bions (MPBs) respectively
  • to supplement 2xYT agar plates to culture BL21 DE3 star cells and BL21dLac Gold cells

Sodium phosphate dibasic solution may be used in the preparation of a wide variety of phosphate based biological buffers for use in cell and molecular biology application.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves


Certificados de análise (COA)

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Protocolos

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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