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I9278

Sigma-Aldrich

Insulina

sterile-filtered, BioXtra, suitable for cell culture

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About This Item

Número CAS:
11061-68-0
Número MDL:
MFCD00131380
Código UNSPSC:
12352209
NACRES:
NA.77
Formulário:
solution
Preço e disponibilidade não estão disponíveis no momento.

esterilidade

sterile-filtered

Nível de qualidade

400

linha de produto

BioXtra

Formulário

solution

concentração

9.5-11.5 mg/mL

técnica(s)

cell culture | mammalian: suitable

Impurezas

≤50 EU/mL endotoxin

pH

7.9-8.5

nº de adesão UniProt

P01308

temperatura de armazenamento

2-8°C

chave InChI

PBGKTOXHQIOBKM-FHFVDXKLSA-N

Informações sobre genes

human ... INS(3630)

Descrição geral

A insulina é um hormônio polipeptídico de duas cadeias produzido pelas células β das ilhotas pancreáticas. Seu peso molecular é de cerca de 5800 Da. As cadeias α e β são ligadas por duas pontes dissulfeto entre as cadeias. A cadeia α contém uma ponte dissulfeto dentro da cadeia.

Aplicação

A solução de insulina humana tem sido usada como suplemento no meio de Eagle modificado (DMEM) de Dulbecco para cultura de linhagem celular epitelial e linhagem celular endometrial humana, meio essencial mínimo Eagle para cultura de linhagem celular de adenocarcinoma de mama humano e meio 199 para cultivar a linhagem de células ovarianas normais.
Solução de insulina humana é recomendada para uso em aplicações de cultura celular em concentração de 0,5 a 1 ml/l por litro de meio.
A solução de insulina humana foi usada para teste de tolerância de insulina em camundongos e ratos.[1][2] Ela também foi usada para diferenciação adipogênica de culturas de crescimento derivadas de células-tronco embrionárias humanas (hESCs) e células estromais mesenquimais humanas (hMSCs).[3][4]

Ações bioquímicas/fisiológicas

A insulina desempenha um papel em vários processos celulares, como promover o crescimento celular, regular o ciclo celular e facilitar a captação de glicose. É comumente usada como suplemento proteico em meios de cultura de células de mamíferos livres de soro para ajudar a promover o crescimento ideal e a manutenção de células de mamíferos.

Outras notas

InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1

forma física

estável a 2-8 °C

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
0000341617
0000327021
0000341617
SLCQ6396
SLCN8981

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Farinaz Ghodrati et al.
Scientific reports, 8(1), 8654-8654 (2018-06-07)
At times, it can be difficult to discern if a lack of overlap in reported interactions for a protein-of-interest reflects differences in methodology or biology. In such instances, systematic analyses of protein-protein networks across diverse paradigms can provide valuable insights.
E Comisso et al.
Oncogene, 36(30), 4253-4266 (2017-03-21)
OCT4 (Octamer-binding transcription factor 4) is essential for embryonic stem cell self-renewal. Here we show that OCT4 increases the aggressiveness of high-grade serous ovarian cancer (HG-SOC) by inactivating the Retinoblastoma tumor suppressor pathway and enhancing mitotic stability in cancer cells.
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Nunes S, et al.
Cardiovascular Diabetology, 12, 44-44 (2013)
Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System.
Jones M et al.
Cytotherapy, 15, 1323-1323 (2013)
Amer Mahmood et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 25(6), 1216-1233 (2010-03-05)
Directing differentiation of human embryonic stem cells (hESCs) into specific cell types using an easy and reproducible protocol is a prerequisite for the clinical use of hESCs in regenerative-medicine procedures. Here, we report a protocol for directing the differentiation of
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Questions

1–10 of 15 Questions  

  1. How is this insulin produced? In E.Coli? Yeast?...?

    1 answer

    1. This product is prepared from recombinant human insulin produced in Saccharomyces cerevisiae.

      Helpful?

  2. Is this product suitable for insulin tolerance tests in mice

    1 answer

    1. This item has not been specifically tested for suitability in insulin tolerance. However, the product has been used successfully in a number of published papers. Please see the link below to review one such article:
      https://pmc.ncbi.nlm.nih.gov/articles/PMC2785638/

      Helpful?

  3. How long does the insulin remain potent for if stored in the fridge?

    1 answer

    1. This product is assigned an expiration date of approximately 2 years from the date of Quality Release, when stored as directed at 2-8°C. The expiration date is lot specific and reported on the product label, as well as the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/i9278#product-documentation

      Helpful?

  4. Is this insulin considered of fast or slow action?

    1 answer

    1. This product is intended for research use only and is not a Therapeutic insulin. The research grade insulin products are not defined by the Therapeutic types. There are analytical standards available for each type. Please see the links below to review additional product options for research use:
      Insulin Glargine - Slow acting Insulin
      https://www.sigmaaldrich.com/search/insulin-glargine?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20glargine&type=product

      Aspart Insulin - Fast acting Insulin
      https://www.sigmaaldrich.com/search/insulin-aspart?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20aspart&type=product

      Lispro Insulin - Fast acting Insulin
      https://www.sigmaaldrich.com/search/insulin-lispro?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20lispro&type=product

      Helpful?

  5. Is this human insulin suitable for use in mouse cell culture?

    1 answer

    1. Yes, this product is suitable for mouse cell culture and has been cell culture tested on the L929 and 3T3 mouse cell lines. Please see the link below to review peer-reviewed articles that may be of interest:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892261/#:~:text=This%20process%20requires%20DRAM%2D1,and%20permitting%20efficient%20mTORC1%20activation.

      Helpful?

  6. what is the morality of the product I9278, Insulin solution human?

    1 answer

    1. The molarity of a 10 mg/mL solution of human insulin, with a molecular weight of 5807 daltons, is approximately 1.7 μmol/mL or 1.7 mM.

      Helpful?

  7. What kind of solution is the insulin (Product number: I9278) dissolved in?

    1 answer

    1. The insulin (cat# I9278) is dissolved in HEPES solution.

      Helpful?

  8. Are there any contaminants (IgG, IgM example) in I9278?

    1 answer

    1. Unfortunately, this product is not tested for impurities.

      Helpful?

  9. What is the content of the insulin solution/buffer (any perservatives and non insulin components?)

    1 answer

    1. This product is prepared in a HEPES buffer, pH 7.9 - 8.5.

      Helpful?

  10. Can you store this product aliquoted at -20 degrees to extend shelf-life?

    1 answer

    1. For research applications, freezing insulin solution has been a common pratice. However, solutions available on the commercial market for therapeutic use are never frozen, in order to maintain maximum activity. There have been no stability studies perform on the shelf life of frozen insulin solutions. Please see the link below to review a publication that may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721649/

      Helpful?

1–10 of 15 Questions  

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