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I1146

Sigma-Aldrich

R3 IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinônimo(s):

Human IGF-1, IGF-1 Protein, Recombinant IGF-1

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About This Item

Código UNSPSC:
51111800
NACRES:
NA.77

fonte biológica

human

Nível de qualidade

200

recombinante

expressed in E. coli

Ensaio

≥85% (HPLC)

forma

lyophilized powder

potência

≤10 ng/mL ED50

qualidade

endotoxin tested

peso molecular

7,676 Da

embalagem

pkg of 0.1 mg

técnica(s)

cell culture | mammalian: suitable

nº de adesão UniProt

P05019

temperatura de armazenamento

2-8°C

Informações sobre genes

human ... IGF1(3479)

Categorias relacionadas

Aplicação

R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

Ações bioquímicas/fisiológicas

Proliferation of chinese hamster ovary CHO cells.

Outras notas

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Nota de preparo

Prepared from 0.1 M acetic acid

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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