10069

α-Amylase aus Bacillus sp.

powder, yellow-brown, ~380 U/mg

• CAS-Nummer: 9000-90-2
• EC-Nummer: 3.2.1.1 (BRENDA, IUBMB)
• EG-Nummer: 232-565-6
• MDL-Nummer: MFCD00081319
• UNSPSC-Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Bacillus sp.
• Qualitätsniveau: 100
• Form: powder
• Spezifische Aktivität: ~380 U/mg
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Farbe: yellow-brown
• Grünere Alternativprodukt-Kategorie: , Enabling
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

α-Amylase (α-1,4-glucan-4-glucanohydrolase) belongs to the glycosyl hydrolase family 13. The two aspartic residues and one glutamic acid residue are the prime catalytic residues of α-amylase.[1] All amylases have three domain regions, namely, domain A with a central (β/α)8 barrel, domain B, and β-structure with a Greek key motif encompassing domain C.[1]

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Anwendung

α-Amylase from Bacillus sp. has been used:

• as a dispersal enzyme to test degradation of S. aureus biofilms[2],
• in the enzymatic hydrolysis of tapioca starch[3]
• in the enzymolysis of plant-based native and the amorphous granular starches[4]

Biochem./physiol. Wirkung

α-Amylase mediates the hydrolysis of starch, malto-oligosaccharides, and glycogen at the α-D-(1,4)-glucosidic linkages.[1] Bacillus sp. serve as an important cell factory for the heterogeneous production of α-amylase.[5] An extracellular secreted thermostable amylase from the Bacillus subtilis strain has also been reported.[6]

Einheitendefinition

1 U entspricht der Enzymmenge, die 1 μmol Maltose pro Minute bei pH 6.9 und 25°C freisetzt (Stärke nach Zulkowsky, Fluka^®-Nr. 85642, als Substrat)

Sonstige Hinweise

Heat stability of bacterial α-amylases[7]; Action pattern on sweet potato starch, amylose and amylopectin[8]; Action on native wheat starch[9]

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves