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Merck

10080

Sigma-Aldrich

α-Amylase aus Schweinepankreas

powder, ~50 U/mg

Synonym(e):

α-Amylase Enzyme

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About This Item

EG-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

hog pancreas

Form

powder

Spezifische Aktivität

~50 U/mg

Lagertemp.

−20°C

Allgemeine Beschreibung

Pancreatic α-amylase is produced by the pancreatic acinar cells and released into the duodenum. It is a major molecule of pancreatic fluid.

Anwendung

α-Amylase from hog pancreas has been used:
  • in the in vitro digestion system to study the hydrolysis of pea globulins
  • in vitro α-amylase inhibition study
  • to prepare digestive juice (salivary juice) for the in vitro model of the human digestion system

Biochem./physiol. Wirkung

α-Amylase is a starch hydrolase, for glucose production, important for energy acquisition. It catalyzes the hydrolysis of α-1,4-glycosidic linkages in starch to form glucose, maltose, and maltotriose units. In mammals, α-amylase regulates the sugar assimilation by reducing the glucose uptake by Na+/glucose cotransporter 1 (SGLT1) and stimulating glycoprotein N-glycans mediated starch digestion. α-Amylase inhibitors are associated with the treatment of type 2 diabetes mellitus. α-Amylases are widely known industrial enzymes used in food, detergent, textile, fermentation, and pharmaceutical industries.

Einheitendefinition

1 U entspricht der Enzymmenge, die 1 μmol Maltose pro Minute bei pH 6.9 und 25°C freisetzt (Stärke nach nach Zulkowsky, Fluka® Nr. 85642, als Substrat)

Sonstige Hinweise

Optimum pH and temperature 6.9 and 53°C, respectively; calcium is required for activity and also prevents degradation by trypsin; Characterization

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Die Dokumentenbibliothek aufrufen

Pieter Van den Abbeele et al.
Nutrients, 12(7) (2020-07-03)
Modulation of the gut microbiome as a means to improve human health has recently gained increasing interest. In this study, it was investigated whether cRG-I, a carrot-derived pectic polysaccharide, enriched in rhamnogalacturonan-I (RG-I) classifies as a potential prebiotic ingredient using
Limited action of trypsin on porcine pancreatic amylase: characterization of the fragments.
M Granger et al.
FEBS letters, 56(2), 189-193 (1975-08-15)
Anna Jakubczyk et al.
BioMed research international, 2014, 438459-438459 (2014-09-25)
Pea seeds represent a valuable source of active compounds that may positively influence health. In this study, the pea globulins were digested in vitro under gastrointestinal condition and potentially bioaccessible angiotensin I converting enzyme (ACE) inhibitory peptides were identified. The
Paula Monteiro de Souza et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 41(4), 850-861 (2010-10-01)
Amylases are one of the main enzymes used in industry. Such enzymes hydrolyze the starch molecules into polymers composed of glucose units. Amylases have potential application in a wide number of industrial processes such as food, fermentation and pharmaceutical industries.
Muhammad H Alu'datt et al.
Food chemistry, 240, 784-798 (2017-09-28)
This investigation was performed to assess the effects of sonication on the structure of protein, extractability of phenolics, and biological properties of isolated proteins and protein co-precipitates prepared from brewers' spent grain and soybean flour. Scanning electron micrographs revealed that

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