Sarcosine Oxidase from Bacillus sp.

Name: Sarcosine Oxidase from <I>Bacillus</I> sp.
Brand: SIGMA

lyophilized powder, 25-50 units/mg solid

Synonym(s):

Sarcosine: oxygen oxidoreductase (demethylating)

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About This Item

CAS Number:

9029-22-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-850-5

MDL number:

MFCD00132221

EC Number:

1.5.3.1 (BRENDA, IUBMB)

Specific activity:

25-50 units/mg solid

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Properties

form

lyophilized powder

Quality Segment

200

specific activity

25-50 units/mg solid

mol wt

44 kDa

storage temp.

−20°C

Description

General description

Monomeric sarcosine oxidase (MSOX) is a flavoenzyme that catalyzes the oxidative demethylation of sarcosine (N-methylglycine) to yield glycine, formaldehyde, and hydrogen peroxide. Monomeric sarcosine oxidase can oxidize other secondary amino acids such as N-methyl-L-alanine, N-ethylglycine, and L-proline. [1]

Sarcosine oxidase is a 44 kDa protein with covalently bound 1 mol of flavin adenine dinucleotide (FAD). It belongs to the family of amino acid oxidases with bound FAD.

Application

Sarcosine oxidase has been used in a study as part of a multienzyme cascade, that when immobilized constructed amperometric biosensors. [2] Sarcosine oxidase has also been used in a study to investigate oxidation of amines by flavoproteins. [3]

Sarcosine oxidase from Bacillus sp. has been used as a positive control for detection of proteins with covalently bound flavin adenine dinucleotide (FAD) by UV. It has also been used in co-immobilization with other enzymes on platinum electrode for preparation of creatinine biosensor.[4]

Physical form

No stabilizers added

Other Notes

One unit will form 1.0 μmole of formaldehyde from sarcosine per min at pH 8.3 at 37 °C.

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This Item	F7296	C4405	228250
Sigma-Aldrich S7897 Sarcosine Oxidase from Bacillus sp.	Sigma-Aldrich F7296 Fructosyl-Amino Acid Oxidase from Corynebacterium sp.	Sigma-Aldrich C4405 Choline Oxidase from Arthrobacter globiformis	Sigma-Aldrich 228250 Cholesterol Oxidase, Streptomyces sp.
specific activity 25-50 units/mg solid	specific activity ≥0.45 units/mg protein	specific activity 8-20 units/mg solid	specific activity ≥3 units/mg solid, ≥40 U/mg
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized solid
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 44 kDa	mol wt ~88 kDa by electrophoresis	mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration	mol wt -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100