P7367

PNGase F from Elizabethkingia meningoseptica

BioReagent, ≥95% (SDS-PAGE)

• Synonym(s): N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase
• CAS Number: 83534-39-8
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD18839214
• Assay: ≥95% (SDS-PAGE)
• Biological source: bacterial (Elizabethkingia meningoseptica)
• Concentration: ≥300 units/mL, ≥50 units/mL

Properties

• biological source: bacterial (Elizabethkingia meningoseptica)
• Quality Segment: 200
• conjugate: (N-linked)
• product line: BioReagent
• assay: ≥95% (SDS-PAGE)
• form: powder
• shelf life: ≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml), ≥1 yr at 2‑8 °C, Solution is stable for at least 3 freeze-thaw cycles
• mol wt: ~36 kDa
• concentration: ≥300 units/mL, ≥50 units/mL
• optimum pH: ~8.6
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Application

PNGase F from Elizabethkingia meningoseptica has been used:

• for de-N-glycosylation of Zika20virus E protein[1]
• to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells[2]
• to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)[3]

Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)
• Storage Class: 11 - Combustible Solids