This product is tested at pH 4.0 at 25C. The optimum pH and temperature have not been determined. However, the publication available at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153499/#:~:text=The%20pectinase%20has%20an%20estimated,temperature%20of%2061%C2%B0C.
P4716
Pectinase from Aspergillus niger
BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)
Synonym(s):
Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-885-6
MDL number:
Specific activity:
≥5 units/mg protein (Lowry)
Biological source:
Aspergillus niger
Pricing and availability is not currently available.
biological source
Aspergillus niger
Quality Segment
product line
BioReagent
form
aqueous glycerol solution
specific activity
≥5 units/mg protein (Lowry)
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
cell culture | plant: suitable
greener alternative category
, Enabling
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Application
Petctinase from Aspergillus niger has been used:
- for protoplasts liberation
- in the protoplast enzyme mixture for enzyme digestion
- to conduct partial saccharification of sugars
- to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Biochem/physiol Actions
Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.
Physical form
Solution in 40% glycerol
Other Notes
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| biological source Aspergillus niger | biological source - | biological source Aspergillus sp. (A. japonicus) | biological source - |
| technique(s) cell culture | plant: suitable | technique(s) - | technique(s) - | technique(s) - |
| specific activity ≥5 units/mg protein (Lowry) | specific activity ≥3,800 units/mL | specific activity ≥0.3 units/mg solid | specific activity 0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate) |
| form aqueous glycerol solution | form aqueous solution | form lyophilized powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Hello! Is 25 degrees Celsius and pH 4.0 the optimal temperature and pH for the highest activity of this enzyme? or do I still need to check? Thanks!
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Helpful?
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