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C0615

Cellulase from Trichoderma sp.

greener alternative

powder, ≥5,000 units/g solid

Synonym(s):

Onozuka RS

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
EC Number:
eCl@ss:
32160410
Specific activity:
≥5,000 units/g solid
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form

powder

Quality Segment

specific activity

≥5,000 units/g solid

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

solubility

deionized water: soluble 3.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB))

greener alternative category

storage temp.

2-8°C

General description

Manufactured by Yakult
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research

Application

Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries.[1] Cellulase is added to detergents to improve cleansing properties.[1] Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose while investigating the biodegradability of bioabsorbable bacterial cellulose (BBC).[2]

Biochem/physiol Actions

Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites [3]. Cellulases have different microbial origins and therefore different protein sequences [1].

Preparation Note

Dissolves in sterile deionized (DI) water in the presence of 0.15 polyhexamethylene biguanide (PHMB) at 3 mg/mL concentration.

Other Notes

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

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This Item
C8546C260522178
specific activity

≥5,000 units/g solid

specific activity

≥1 unit/mg solid

specific activity

≥1000 units/g

specific activity

~0.8 U/mg

form

powder

form

lyophilized powder

form

solid

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

deionized water: soluble 3.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB))

solubility

deionized water: soluble 5.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).)

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

-


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Global Trade Item Number

SKUGTIN
C0615-1G04061835555765

Questions

1–4 of 4 Questions  
  1. What is the working pH and temperature, and the optimal pH and temperature, please?

    1 answer
    1. We have not tested pH on this and do not have a working temperature range on this product.  However, we do mention: One unit will liberate 1.0 micromole of glucose from cellulose per hour at pH 5.0 at 37 deg C (2 hour assay).  

      Helpful?

  2. ¿What cellulases does the lyophilized powder C0615 contain? and ¿what is the ratio?

    1 answer
    1. This product is a high purity, high activity cellulase derived from Trichoderma viride. An isoenzyme profile has not been performed. The link below may be useful:
      https://febs.onlinelibrary.wiley.com/doi/full/10.1111/j.1432-1033.1985.tb08653.x

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Does product C0615, cellulase from Trichoderma viride, contain any sugar as a stabilizer?

    1 answer
    1. No. Product C0615, cellulase from Trichoderma viride, does not contain any sugar stabilizer.

      Helpful?

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