This product is assigned an activity specification of 30 - 60 units per milligram protein. The minimum protein content is 80%. At the minimum protein content, each 5 mg vial will contain 120 - 240 units. This information is lot-specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a7420#product-documentation
A7420
Amyloglucosidase from Aspergillus niger
lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose
Synonym(s):
1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-877-2
MDL number:
Specific activity:
30-60 units/mg protein (biuret)
Pricing and availability is not currently available.
form
lyophilized powder
Quality Level
specific activity
30-60 units/mg protein (biuret)
composition
protein, ≥80%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
impurities
≤0.02% glucose
greener alternative category
, Enabling
storage temp.
−20°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle [1].
Biochem/physiol Actions
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides.[2] Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.[3]
Physical form
Lyophilized powder containing less than 0.02% glucose
Other Notes
One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| specific activity 30-60 units/mg protein (biuret) | specific activity ~70 U/mg | specific activity ~120 U/mg | specific activity ≥40 units/mg protein |
| form lyophilized powder | form powder | form powder | form ammonium sulfate suspension |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| impurities ≤0.02% glucose | impurities - | impurities - | impurities - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| greener alternative category , Enabling | greener alternative category , Enabling | greener alternative category , Enabling | greener alternative category - |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Yi-Chen Lin et al.
PloS one, 12(5), e0177115-e0177115 (2017-05-05)
It has been proposed that malto-oligosaccharides (MOSs) are possibly recycled back into amylopectin biosynthesis via the sequential reactions catalyzed by plastidial α-glucan phosphorylase 1 (Pho1) and disproportionating enzyme 1 (Dpe1). In the present study, the reciprocal co-immunoprecipitation experiments using specific
Youngmi Kim et al.
Bioresource technology, 99(12), 5165-5176 (2007-11-09)
DDGS and wet distillers' grains are the major co-products of the dry grind ethanol facilities. As they are mainly used as animal feed, a typical compositional analysis of the DDGS and wet distillers' grains mainly focuses on defining the feedstock's
Erick Perera et al.
Scientific reports, 10(1), 18676-18676 (2020-10-31)
The aim of this work was to evaluate two functional feeds for the gilthead seabream, Sparus aurata, containing low inclusion of two microalgae-based products (LB-GREENboost, LBGb; and LB-GUThealth, LBGh). Fish (12-13 g) were fed for 13 weeks a control diet or one
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A7420-25MG | 04061835553648 |
| A7420-5MG | 04061835540631 |
| A7420-100MG | 04061835540624 |
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How do you determine how many units there are in the sample I have, or how much substrate it will break down?
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What is the shelf life of a solution (1 mg/mL in water) of Product A7420, Amyloglucosidase from Aspergillus niger and how should it be stored?
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The stability of this product in solution has not been evaluated. However, 172426 Glucoamylase (syn. Amyloglucosides) from Rhizopus, notes that solutions are stable when stored frozen in aliquots at -20°C for up to 3 months. Similar stability would be expected for A7420. Avoid freeze/thaw cycles of solutions.
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Does Product A7420, Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?
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The starting material for Product No. A7420 is Product No. A3042, which is a preparation of the enzyme in a 1 M glucose solution. This is dialyzed against water until the glucose concentration is less than 0.02%, and then lyophilized.
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How can a solution of Product A7420, Amyloglucosidase from Aspergillus niger, be prepared?
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A 1 mg/mL solution may be prepared in water.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Product A7420, Amyloglucosidase from Aspergillus niger?
1 answer-
Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).
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