73009

ECC ChromoSelect Agar

NutriSelect^® Plus, suitable for microbiology

• Synonym(s): E. coli Coliform Agar ChromoSelect, ECC Agar ChromoSelect
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; chromogenic mixture, 20.3 g/L ; disodium hydrogen phosphate, 3.5 g/L ; lactose, 2.5 g/L ; monopotassium hydrogen phosphate, 1.5 g/L ; neutral red, 0.03 g/L ; peptone, special, 5 g/L ; sodium chloride, 5 g/L ; yeast extract, 3 g/L
• manufacturer/tradename: NutriSelect^® Plus
• final pH: 6.8±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; water monitoring; microbiology
• suitability: nonselective and differential for Enterobacter spp.; nonselective and differential for Escherichia coli; nonselective and differential for Klebsiella spp.; nonselective and differential for coliforms

Description

Application

ECC ChromoSelect Agar is a differential medium recommended for the presumptive identification of Escherichia coli and other coliforms in food and environmental samples. The chromogenic mixture containstwo chromogens as X-glucuronide and Salmon-GAL. X-glucuronide is cleaved by the enzyme beta glucuronidase produced by Escherichia coli. Salmon-GAL is cleaved by the enzyme galactosidase produced by majority of coliforns, including Escherichia coli. Color of E. coli colonies: blue/purple

Preparation Note

Suspend 55.8 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable