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A4550

Agar

Type A, suitable for plant cell culture

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
UNSPSC Code:
50405901
eCl@ss:
42040102
EC Number:
232-658-1
NACRES:
NA.21
MDL number:
MFCD00081288
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biological source

algae (Rhodophyceae)

Quality Level

200

type

Type A

form

powder

technique(s)

cell culture | plant: suitable

transition temp

transition temp 32-38 °C (1.5%)

gel strength

>650 g/cm2

application(s)

agriculture

storage temp.

room temp

SMILES string

O1[C@@H]([C@@H]([C@@H]([C@H](C1OC2[C@H]3OCC2OC([C@H]3O)C)O)OC)O)CO

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

General description

Agar (Type A) is extracted from agarocytes of the red alga Rhodophyceae. It is a polysaccharide complex, consisting of a neutral gelling fraction, agarose, and a non-gelling fraction, agaropectin. Type-A agar forms a thermoreversible gel, and is most suitable for plant cell culture.

Application

Type A agar has been used:
  • to perform metal(loid)-related phenotyping of the Arabidopsis thaliana metal-sensitive mutant strains[1]
  • as a component of Murashige and Skoog (MS) media to culturing Arabidopsis thaliana seeds[2]
  • to culture Cicer arietinum L. (Chickpea) seeds for Agrobacterium rhizogenes mediated root transformation[3]

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This Item
A66860503805039
Agar Type A, suitable for plant cell culture

Sigma-Aldrich

A4550

Agar

Agar Bacteriological, microbiology tested, suitable for plant cell culture, granular

Sigma-Aldrich

A6686

Agar

Agar suitable for microbiology

Millipore

05038

Agar

Agar suitable for microbiology

Millipore

05039

Agar

form

powder

form

granular

form

powder

form

powder

gel strength

>650 g/cm2

gel strength

-

gel strength

>900 g/cm2

gel strength

>800 g/cm2 (1.5% gel, Nikkan, after autoclaving)

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

-

technique(s)

-

biological source

algae (Rhodophyceae)

biological source

algae (Rhodophyceae)

biological source

-

biological source

-

type

Type A

type

-

type

-

type

-

transition temp

transition temp 32-38 °C (1.5%)

transition temp

transition temp 32-39 °C

transition temp

gel point 32-35 °C

transition temp

gel point 33-37 °C (1.5% solution, after autoclaving)

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Storage Class

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Certificates of Analysis (COA)

Lot/Batch Number
0000552800
0000550641
0000550641
0000529008
0000529008

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Antoine Desrut et al.
Journal of experimental botany, 71(22), 7301-7315 (2020-08-30)
Plants live in close relationships with complex populations of microorganisms, including rhizobacterial species commonly referred to as plant growth-promoting rhizobacteria (PGPR). PGPR are able to improve plant productivity, but the molecular mechanisms involved in this process remain largely unknown. Using
Zhihang Feng et al.
Plant physiology, 184(1), 428-442 (2020-07-01)
Mg2+ is among the most abundant divalent cations in living cells. In plants, investigations on magnesium (Mg) homeostasis are restricted to the functional characterization of Mg2+ transporters. Here, we demonstrate that the splicing factors SUPPRESSORS OF MEC-8 AND UNC-52 1
Shimpei Uraguchi et al.
Frontiers in plant science, 11, 503-503 (2020-06-06)
For researchers in the plant metal field, the agar reagent used for the solid plate medium is a problematic factor because application of different agar types and even a different lot of the same agar type can mask the plant
View All Related Papers

Global Trade Item Number

SKUGTIN
A4550-5KG04061835542161
A4550-500G04061833374610
A4550-1KG04061835542154

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