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A8887

Acrylamide

suitable for electrophoresis, ≥99%

Synonym(s):

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
NACRES:
NB.22
PubChem Substance ID:
eCl@ss:
39031205
UNSPSC Code:
41105319
EC Number:
201-173-7
MDL number:
Beilstein/REAXYS Number:
605349
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vapor density

2.45 (vs air)

Quality Level

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99%

form

crystals

technique(s)

electrophoresis: suitable

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

suitability

suitable for electrophoresis

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

General description

Acrylamide acts as a quencher of tryptophan fluorescence. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis. Bis-acrylamide is required for crosslinking and acrylamide is vital for the polymerization of monomers. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules.
Acrylamide (AA) is a neurotoxin and carcinogen. Potato products like, French fries, potato chips and also several cereal-based products contains acrylamide. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.

Application

Acrylamide has been used:
  • for the synthesis of hydrogels
  • for the preparation of polyacrylamide gel[1]
  • to agglomerate vimentin and keratin intermediate filament network

Acrylamide has been used:
  • to agglomerate the vimentin and keratin intermediate filament network of K562cells
  • in the preparation and characterization of soft culture substrate[1]
  • in hydrogel synthesis
  • for determination of protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis


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This Item
A35530169608267
form

crystals

form

powder

form

powder blend

form

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

suitability

suitable for electrophoresis

suitability

-

suitability

-

suitability

-

bp

125 °C/25 mmHg (lit.)

bp

125 °C/25 mmHg (lit.)

bp

125 °C/25 mmHg (lit.)

bp

125 °C/25 mmHg (lit.)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges



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Related Content

Product Information Sheet


Voltage-induced wrinkle performance in a hydrogel by dielectric elastomer actuation
Tang C, et al.
Polymer, 10(7), 697-697 (2018)
Matrix stiffness modulates formation and activity of neuronal networks of controlled architectures
Lantoine J, et al.
Biomaterials, 89, 14-24 (2016)
Use of potato flour in bread and flat bread
Flour and Breads and Their Fortification in Health and Disease Prevention, 247-259 (2011)



Global Trade Item Number

SKUGTIN
A8887-2.5KG04061835542611
A8887-100G04061835542598
A8887-500G04061835542628
A8887-1KG04061835542604
A8887-5G04061833425961

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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