RAB0508

Human VEGF ELISA Kit

for cell and tissue lysates

• Synonym(s): VEGF-A, Vascular endothelial growth factor A
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... VEGFA(7422)
• Input: sample type cell lysate; sample type tissue lysate
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type cell lysate; sample type tissue lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 10 pg/mL; standard curve range: 8.23-6000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... VEGFA(7422)

Description

General description

The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in cell lysate and tissue lysate.

Immunogen

Recombinant Human VEGF-A

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis. Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes. Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease. Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABDIL6

  ELISA 5X Sample Diluent Buffer (Item D2)

  SDS
• RABELADE

  ELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  SDS
• RABLYSIS1

  2X Cell Lysis Buffer (Item J)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 10 - Combustible liquids