This product is not dephosphorylated. For dephosphorylated options, consider product C4032, Casein, which is dephosphorylated using potato acid phosphatase.
C5890
Casein from bovine milk
suitable for substrate for protein kinase (after dephosphorylation), purified powder
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About This Item
Form:
purified powder
Biological source:
bovine milk
Pricing and availability is not currently available.
Quality Segment
biological source
bovine milk
form
purified powder
technique(s)
activity assay: suitable, electrophoresis: suitable, immunocytochemistry: suitable
mp
280 °C (dec.) (lit.)
suitability
suitable for substrate for protein kinase (after dephosphorylation)
SMILES string
[P](=O)(OCC(NC(=O)C(NC(=O)C(N)Cc1ccccc1)CCC(=O)N)C(=O)NC(CCC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CCC(=O)N)C(=O)NC(CCC(=O)N)C(=O)NC(CCC(=O)N)C(=O)NC(C(O)C)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)N)C(=O)NC(CC(=O)O)C(=O)NC(C
InChI
1S/C81H125N22O39P/c1-36(2)31-50(76(132)94-43(15-24-57(87)108)71(127)101-52(34-64(120)121)78(134)98-49(81(137)138)11-7-8-30-82)99-72(128)47(19-28-61(114)115)95-77(133)51(33-63(118)119)100-73(129)48(20-29-62(116)117)97-80(136)65(37(3)104)103-75(131)44(16-25-58(88)109)92-68(124)42(14-23-56(86)107)90-67(123)41(13-22-55(85)106)91-69(125)45(17-26-59(110)111)93-70(126)46(18-27-60(112)113)96-79(135)53(35-142-143(139,140)141)102-74(130)40(12-21-54(84)105)89-66(122)39(83)32-38-9-5-4-6-10-38/h4-6,9-10,36-37,39-53,65,104H,7-8,11-35,82-83H2,1-3H3,(H2,84,105)(H2,85,106)(H2,86,107)(H2,87,108)(H2,88,109)(H,89,122)(H,90,123)(H,91,125)(H,92,124)(H,93,126)(H,94,132)(H,95,133)(H,96,135)(H,97,136)(H,98,134)(H,99,128)(H,100,129)(H,101,127)(H,102,130)(H,103,131)(H,110,111)(H,112,113)(H,114,115)(H,116,117)(H,118,119)(H,120,121)(H,137,138)(H2,139,140,141)
InChI key
BECPQYXYKAMYBN-UHFFFAOYSA-N
Gene Information
bovine ... CSN1S1(282208), CSN1S2(282209), CSN2(281099), CSN3(281728)
General description
Casein from bovine milk is a phosphoprotein. There are four main types of Casein which make up approximately 80% of the total protein in bovine milk: α-s1 Casein, α-s2 Casein, β-Casein, and κ-Casein. Casein is proposed to be the main protective constituent in milk.
Application
Casein from bovine milk has been used:
- to prepare casein hydrolysates
- as a calibrating substance in biuret colorimetric method
- as a supplement in nutrient agar for the determination of protease activity
- to prepare protein-enriched diet for independent replicate lines of D. melanogaster
Biochem/physiol Actions
Partial gastrointestinal digestion of casein is a rich source of bioactive peptides, such as β-casomorphin. However, bovine casein is not homogeneous; variants A1 and B do lead to production of β-casomorphin 7 production, while A2 does not.
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This Item | |||
|---|---|---|---|
| biological source bovine milk | biological source bovine milk | biological source - | biological source bovine milk |
| technique(s) activity assay: suitable, immunocytochemistry: suitable, electrophoresis: suitable | technique(s) activity assay: suitable, electrophoresis: suitable, immunocytochemistry: suitable | technique(s) - | technique(s) activity assay: suitable, electrophoresis: suitable, immunocytochemistry: suitable |
| suitability suitable for substrate for protein kinase (after dephosphorylation) | suitability - | suitability - | suitability - |
| form purified powder | form powder | form - | form powder |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. |
| Gene Information bovine ... CSN1S1(282208), CSN1S2(282209), CSN2(281099), CSN3(281728) | Gene Information bovine ... CSN1S1(282208), CSN1S2(282209), CSN2(281099), CSN3(281728) | Gene Information - | Gene Information bovine ... CSN1S1(282208), CSN1S2(282209), CSN2(281099) |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
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How this product has been dephosphorylated ? (Acidic phosphatase, basic phosphatase...)
1 answer-
Helpful?
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Helpful?
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What is the composition of Product C5890, Casein from bovine milk?
1 answer-
The approximate casein composition of milk in grams per Liter is: alpha-s1 (12-15); alpha-s2 (3-4); beta (9-11); and kappa (2-4).
Helpful?
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What is Product C5890, Casein from bovine milk, soluble in?
1 answer-
Casein is not water soluble. It can be suspended in 1 M sodium hydroxide at a concentration of 50 mg/mL, yielding a turbid, faint yellow solution.
Helpful?
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What is the molecular weight of the casein subunits in Product C5890, Casein from bovine milk?
1 answer-
The alpha-s1 = 22-23.7 kDa, alpha-s2 = 25 kDa, beta = 24 kDa and kappa = 19 kDa.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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