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T0167
D-(+)-Trehalose dihydrate
from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
Synonym(s):
α,α-Trehalose, α-D-Glucopyranosyl-α-D-glucopyranoside
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About This Item
Empirical Formula (Hill Notation):
C12H22O11 · 2H2O
CAS Number:
Molecular Weight:
378.33
UNSPSC Code:
12352201
NACRES:
NA.75
PubChem Substance ID:
EC Number:
202-739-6
Beilstein/REAXYS Number:
5322018
MDL number:
Pricing and availability is not currently available.
Quality Segment
biological source
Saccharomyces cerevisiae
product line
BioReagent
assay
≥99%
form
powder
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable
solubility
H2O: 50 mg/mL
SMILES string
[H]O[H].[H]O[H].OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
InChI
1S/C12H22O11.2H2O/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12;;/h3-20H,1-2H2;2*1H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-;;/m1../s1
InChI key
DPVHGFAJLZWDOC-PVXXTIHASA-N
General description
Trehalose is a non-reducing disaccharide, ubiquitously present in nature. It is used as a food preservative and also to retain enzymes, vaccines, cells and many other molecules. Trehalose is present as hemolymph sugar among the invertebrates.
Application
D-(+)-Trehalose dihydrate has been used in a slow-freezing method for freezing rabbit oocytes. It has also been used in solid-surface vitrification (SSV) of in vitro-matured oocytes. It is suitable for use as a cryoprotectant in a variety of cell freezing media.
Biochem/physiol Actions
Trehalose is essential for embryogenesis, carbon metabolism, photosynthesis and flowering in plants. It predominantly exists among the species that supports anhydrobiosis (life without water). It serves as a carbohydrate reserve in microorganisms and protects them from adverse conditions, such as heat, cold, dehydration, desiccation and oxidation. Trehalose acts as a chemical chaperone affecting protein folding. It is known to prevent amyloid generation and accummulation of β-amyloid in vitro. A similar effect of trehalose has been observed with huntingtin protein aggregation. Thus, it might serve as a protective agent in Alzheimer′s disease and Huntington′s disease.
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This Item | |||
|---|---|---|---|
| assay ≥99% | assay ≥99% (HPLC) | assay ≥99% (HPLC) | assay ≥98.5% (HPLC) |
| biological source Saccharomyces cerevisiae | biological source Saccharomyces cerevisiae | biological source Manihot esculenta root (Cassava) | biological source Saccharomyces cerevisiae |
| technique(s) cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) HPLC: suitable, PCR: suitable, cell based assay: suitable, cryopreservation: suitable | technique(s) HPLC: suitable | technique(s) HPLC: suitable, cryopreservation: suitable |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 300 |
| form powder | form powder | form powder | form powder |
| product line BioReagent | product line - | product line - | product line - |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the average particle size in microns or particle mesh size for this product, please? This is important for my research work.
1 answer-
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We would like to dissolve trehalose in water in bulk and store it. Does dissolved trehalose loose its stability after an extended storage time? Which storage temperature would you recommend?
1 answer-
The solution stability and storage of this product has not been tested, however other commercial sources offer 1M Trehalose solutions that are stored at ambient temperature or refrigerated (filter-sterilized). Trehalose is added to several of less stable protein preparations as a carrier and/or cryoprotectant. An example would be EGFR, E2645. This lyophilized product contains 10% Trehalose. Upon reconstitution the product can be stored for up to 1 year at -70 deg C. Given the information above, Trehalose may be quite stable in solution.
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Can solutions of Product T0167, D-(+)-Trehalose dihydrate, be autoclaved?
1 answer-
Do not autoclave. It is best to sterile filter solutions.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product T0167, D-(+)-Trehalose dihydrate, be used as a substitute for protein in a freezing media?
1 answer-
Yes, it should work as a substitute for protein in a freezing media. It is commonly used at a final concentration of 5% (50 mg/mL). Alternatively, one could use methylcellulose.
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Do we have information of any fluorescent impurities in Product T0167, D-(+)-Trehalose dihydrate?
1 answer-
This product is not tested for the presence or absence of fluorescent impurities.
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