M8403

McCoy’s 5A Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): 5A basal medium
• UNSPSC Code: 12352207
• NACRES: NA.75
• EC Number: 200-655-4
• MDL number: MFCD00217560

Properties

• Product Name: McCoy′s 5A Medium, Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: no; NaHCO₃: 2.2 g/L; glucose: 3.0 g/L; phenol red: 0.011 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

McCoy′s 5A Medium is suitable for the primary culture of cells from a wide range of tissue. It is recommended for the production of viruses in primary cell cultures.

Application

McCoy′s 5A Medium has been used to maintain:

• G-361 melanoma cells;
• 87-MG cells
• human colon carcinoma cell line HCT116

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Preparation Note

Supplement with 0.22 g/L L-glutamine.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable