CS0760

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

• UNSPSC Code: 12352207
• EC Number: 217-896-6
• NACRES: NA.32
• Technique(s): flow cytometry: suitable, protein staining: suitable
• Usage: kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette)
• Storage temp.: −20°C
• Detection method: fluorometric
• Shipped in: dry ice

Properties

• Quality Level: 200
• usage: kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette)
• packaging: pkg of 1 kit
• storage condition: dry at room temperature
• technique(s): flow cytometry: suitable, protein staining: suitable
• fluorescence: λ_ex 490 nm; λ_em 590 nm (JC-1 dye)
• application(s): cell analysis; detection
• detection method: fluorometric
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.[1]

Biochem/physiol Actions

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Features and Benefits

• Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
• Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
• Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Analysis Note

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Kit Components Only

• DMSO 1 mL
• JC-1 Assay Buffer 5X 25 mL
• JC-1 Stain 25 μg
• Valinomycin Ready Made 100 μL

Safety Information

• pictograms: GHS06
• signalword: Danger
• hcodes: H300 + H310
• pcodes: P262 - P264 - P280 - P301 + P310 - P302 + P352 + P310 - P361 + P364
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 1 Oral
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3