B6032

BIS-TRIS hydrochloride

≥99.0% (titration)

• Synonym(s): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane
• Empirical Formula (Hill Notation): C₈H₁₉NO₅ · HCl
• CAS Number: 124763-51-5
• Molecular Weight: 245.70
• NACRES: NA.25
• PubChem Substance ID: 24891891
• UNSPSC Code: 12161700
• MDL number: MFCD00171252

Properties

• Quality Level: 200
• assay: ≥99.0% (titration)
• form: crystalline powder
• useful pH range: 5.8-7.2
• pKa: 6.5
• solubility: water: 0.5 g/mL, clear, colorless to very faintly yellow
• application(s): diagnostic assay manufacturing
• storage temp.: room temp
• SMILES string: Cl.OCCN(CCO)C(CO)(CO)CO
• InChI: 1S/C8H19NO5.ClH/c10-3-1-9(2-4-11)8(5-12,6-13)7-14;/h10-14H,1-7H2;1H
• InChI key: VEYRVLHAMHQVTC-UHFFFAOYSA-N

Description

Application

• Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions.: This research introduces the use of BIS-TRIS hydrochloride in Phos-tag SDS-PAGE systems to profile protein phosphorylation. The system operates under neutral pH conditions, making it suitable for analyzing proteins with diverse molecular weights. This method enhances the resolution of phosphorylated proteins, facilitating detailed phosphorylation studies crucial for understanding cellular signaling and protein function (Kinoshita et al., 2012).

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable