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Pepsin from porcine gastric mucosa

Name: Pepsin from porcine gastric mucosa
Brand: SIGMA

powder, slightly beige, ≥500 U/mg

Synonym(s):

Pepsin A, Pepsin from hog stomach

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About This Item

CAS Number:

9001-75-6

UNSPSC Code:

12352204

eCl@ss:

42010127

EC Number:

232-629-3

NACRES:

NA.54

MDL number:

MFCD00081840

EC Number:

3.4.23.1 (BRENDA, IUBMB)

Specific activity:

≥500 U/mg

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Properties

form

powder

Quality Level

200

specific activity

≥500 U/mg

mol wt

35 kDa

color

slightly beige

UniProt accession no.

P00791

storage temp.

2-8°C

Gene Information

pig ... LOC396892(396892)

Description

Application

Pepsin cleavage can be used to produce F(ab′)₂ fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Other Notes

One unit corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37 °C (Hemoglobin, Catalog No. 51290, as substrate); 15,000 absorbance units as described above are equivalent to ∼1 Bergmeyer unit. One Bergmeyer unit is the amount of enzyme which hydrolyzes 1 μmole Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37 °C.

Peptide synthesis,citationcitation Review.citation

Peptide synthesis,[1] Review.

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This Item	P7000	SRE0001	516360
Sigma-Aldrich 77160 Pepsin from porcine gastric mucosa	Sigma-Aldrich P7000 Pepsin from porcine gastric mucosa	Sigma-Aldrich SRE0001 Pepsin from porcine gastric mucosa	Sigma-Aldrich 516360 Pepsin, Porcine Stomach Mucosa
specific activity ≥500 U/mg	specific activity ≥250 units/mg solid	specific activity ≥3200 units/mg protein	specific activity ≥2000 units/mg protein
Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)	Gene Information -
form powder	form powder	form lyophilized powder	form lyophilized
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
mol wt 35 kDa	mol wt 35 kDa	mol wt 35 kDa	mol wt -
UniProt accession no. P00791	UniProt accession no. P00791	UniProt accession no. P00791	UniProt accession no. -

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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J.S. Fruton

Carlsberg Research Communications, 49, 41-41 (1984)

Synthesis of tetrapeptide p-nitroanilides catalyzed by pepsin.

C A Abdel Malak et al.

International journal of peptide and protein research, 41(2), 97-101 (1993-02-01)

Swine pepsin at pH 5 efficiently catalyzes a condensation between Z-Ala-Ala-Phe-OH and p-nitroanilides of Leu, Phe, Val, Ala and Arg that leads to formation of corresponding benzyloxycarbonyl-tetrapeptide p-nitroanilides with yields of 70-90%. These reactions are complicated by co-precipitation of pepsin

M.P. Bemquerer et al.

Biomedica Biochimica Acta, 50 (1991)

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Global Trade Item Number

SKU	GTIN
77160-100G	04061833311936

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