68059

FITC-CM-Dextran

average mol wt 4,000

• Synonym(s): FITC-CM-Dextran 4, Fluorescein isothiocyanate–Carboxymethyl–Dextran, Fluorescein isothiocyanate–Carboxymethyl–Dextran
• NACRES: NA.32
• UNSPSC Code: 12352116

Properties

• biological source: synthetic
• Quality Segment: 100
• conjugate: FITC conjugate
• form: powder or crystals
• mol wt: average mol wt 4,000
• composition: carboxymethyl content, 3-7%
• extent of labeling: 0.001-0.020 mol/mol FITC glucose
• technique(s): permeability assay: suitable
• color: light orange to dark orange, orange-yellow, light yellow to dark yellow, yellow-orange
• fluorescence: λ_ex 493 nm; λ_em 517 nm±5 nm in 0.1 M Tris pH 8.0

Description

General description

FITC-CM-Dextran, also known as Fluorescein isothiocyanate–Carboxymethyl–Dextran, is an anionic fluorescent probe. The carboxyl group provides the negative charge, which improves the overall permeability of the molecule. It is supplied as a yellow powder that is freely soluble in water and electrolyte solutions. FITC-CM-Dextran is insoluble in most organic samples such as ethanol, methanol, acetone, and chloroform. FITC-CM-Dextran is manufactured by reacting selected dextran fractions with an activated carboxymethyl derivative in alkali, whereby O-carboxymethyl groups are introduced along the dextran chain.

Application

FITC-CM-dextran is used to study drug intake and delivery mechanisms in HeLa cell lines. FITC-CM-Dextran was chosen due to its high water solubility and negative charge. [1] It is also a suitable molecule to be used in encapsulation studies for polymersomal drug delivery systems or Ps DDS.[2] FITC-CM-Dextran is a biocompatible molecule used as a starting material in several pharmaceutical and diagnostic applications.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable