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P1435

Phosphatase, Acid from sweet potato

Name: Phosphatase, Acid from sweet potato
Brand: SIGMA

ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)

Sinónimos:

(Orthophosphoric-monoester phosphohydrolase [acid optimum] )

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Número CAS:

9001-77-8

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-630-9

MDL number:

MFCD00131847

Número CE:

3.1.3.2 (BRENDA, IUBMB)

eCl@ss:

42010102

Specific activity:

≥10.0 units/mg protein (modified Warburg-Christian)

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Propiedades

form

ammonium sulfate suspension

Quality Segment

300

specific activity

≥10.0 units/mg protein (modified Warburg-Christian)

secondary activity

≤9.9 units/mg protein ATPase

mol wt

110 kDa

storage temp.

2-8°C

SMILES string

O(CC(O)C)CC#C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

Descripción

General description

Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points. [1]

Application

Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase. [2]

Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. [3] It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase.

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO₄ + H₂O --------------- R-OH + HOPO₃ ²⁺Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes.

Physical form

Suspension in 1.8 M (NH₄)₂SO₄, 10 mM MgCl₂, pH 5.3

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

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Este artículo	SRE0048	P1146	P3752
Sigma-Aldrich P1435 Phosphatase, Acid from sweet potato	Sigma-Aldrich SRE0048 Phosphatase, Acid from potato	Sigma-Aldrich P1146 Phosphatase, Acid from potato	Sigma-Aldrich P3752 Phosphatase, Acid from potato
specific activity ≥10.0 units/mg protein (modified Warburg-Christian)	specific activity 3.0-10.0 unit/mg solid	specific activity ≥3.0 units/mg solid	specific activity ≥0.5 unit/mg solid
form ammonium sulfate suspension	form lyophilized powder	form lyophilized powder	form lyophilized powder
mol wt 110 kDa	mol wt -	mol wt 69 kDa	mol wt 69 kDa
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 300	Quality Level 400	Quality Level 300	Quality Level 200
secondary activity ≤9.9 units/mg protein ATPase	secondary activity -	secondary activity -	secondary activity -