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P8165

Phosphate Buffer pH 6.6 at 25 °C

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About This Item

NACRES:
NA.47
UNSPSC Code:
12352107
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form

powder

Quality Segment

color

white

pH

6.60-6.80

application(s)

hematology
histology

storage temp.

room temp

General description

Phosphate buffer serves as an important fixative for light and electron microscopy.

Application

Phosphate buffer is the most commonly used component of irrigation solution during surgeries, to preserve living tissues. It is also used in immunohistochemical staining procedures.

Preparation Note

Each vial will prepare 3.8L of buffer, 34mM.

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This Item
1805P3288F430-99
Quality Level

200

Quality Level

500

Quality Level

200

Quality Level

300

form

powder

form

powder

form

powder

form

liquid

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

food and beverages
pharmaceutical

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

2-25°C

pH

6.60-6.80

pH

7.60-7.80 (25 °C)

pH

7.10-7.30

pH

7.2±0.2 (25 °C)

color

white

color

-

color

white

color

-


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–3 of 3 Questions  
  1. Could I use this buffer to make a 0,5 M solution?

    1 answer
    1. The product's Instructions for Use call for it to be dissolved in 3.8 liters of water. The resulting pH should be between 6.60 to 6.80. The combined molarity of the salts in 3.8 liters is 34 mM. No attempts have been documented for dissolving P8165 to a other concentrations. The individual salts in the formulation are tested for solubility at 100 mg/ml. As such, it is possible a 0.5M solution can be prepared. Mix for an extended period of time before checking the pH. The pH of a 0.5M solution has not been measured.

      Helpful?

  2. En cuanta cantidad de agua destilada se tiene que disolver el producto de cada vial?

    1 answer
    1. Each vial of P8165-1vl should be dissolved in 3.8 liters of deionized water.

      Helpful?

  3. What is the weight of the product? Is it 35.3g?

    1 answer
    1. The weight of the individual vials is not recorded. Although the weights for random bottles are determined, the weights for each vial are not recorded. It is expected that each vial should contain approximately 17.5 grams, and anything between 17.5 to 18 grams would be considered acceptable.

      Helpful?

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