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L4130

Lipopolysaccharides from Escherichia coli O111:B4

purified by trichloroacetic acid extraction

Synonym(s):

LPS

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About This Item

CAS Number:
UNSPSC Code:
12352201
EC Number:
297-473-0
NACRES:
NA.25
MDL number:
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biological source

Escherichia coli (O111:B4)

Quality Segment

form

lyophilized powder

purified by

trichloroacetic acid extraction

impurities

1-10% Protein (Lowry)

color

white to light yellow

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

General description

This product is TCA extracted from E. coli serotype O111:B4. The source strain is from a private collection. This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Show Differences

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This Item
L3012L4005L3755
biological source

Escherichia coli (O111:B4)

biological source

Escherichia coli (O111:B4)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O26:B6)

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

white to light yellow

color

white to faint yellow

color

white to yellow

color

white to yellow


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pictograms

Skull and crossbones

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Global Trade Item Number

SKUGTIN
L4130-100MG04061833957288
L4130-500MG04061833272428

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hi, The LPS (L4130) product data sheet does not include at what temperature it should be stored after dissolving with water or PBS. Can you help me with this issue, please?

    1 answer
    1. Please see the following link describing our LPS products: https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      Solutions at 1 mg/mL in buffer or culture medium are stable for approximately one month at 2-8 °C. For long term storage, frozen aliquots are stable up to 2 years. Avoid repeated freeze/thaw cycles. Solutions should be stored in silanized containers as LPS can bind to plastics and certain types of glass, particularly at concentrations of <0.1 mg/mL. If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is negligible. If glass containers are used, solutions should be vortexed for at least 30 minutes to redissolve any adsorbed product.

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