This stock solution may be diluted with sterile water, medium, or an aqueous buffer such as HEPES or phosphate-buffered saline. Sterile saline is also acceptable for dilution.
I0516
Insulin solution from bovine pancreas
BioReagent, 10 mg/mL insulin in 25 mM HEPES, pH 8.2, sterile-filtered, suitable for cell culture
Synonym(s):
Bovine insulin, Cell culture grade insulin
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About This Item
Biological source:
bovine
Form:
solution
Pricing and availability is not currently available.
biological source
bovine
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
solution
concentration
10 mg/mL insulin in 25 mM HEPES, pH 8.2
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
impurities
endotoxin, tested
pH
7.9-8.5
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
SMILES string
SC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CN)[C@H](CC)C)C(C)C)CCC(=O)O)CCC(=O)N)CS)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc4ccc(cc4)O)C(=O)N[
InChI
1S/2C97H151N25O34S4/c2*1-12-46(10)77(120-72(131)35-98)96(154)122-76(45(8)9)94(152)107-55(24-28-74(134)135)79(137)104-53(22-26-69(100)128)82(140)116-66(40-159)93(151)118-64(38-157)90(148)103-47(11)78(136)114-63(37-124)89(147)121-75(44(6)7)95(153)119-67(41-160)92(150)115-62(36-123)88(146)109-57(30-43(4)5)84(142)110-58(31-48-13-17-50(125)18-14-48)85(143)105-52(21-25-68(99)127)80(138)108-56(29-42(2)3)83(141)106-54(23-27-73(132)133)81(139)112-60(33-70(101)129)87(145)111-59(32-49-15-19-51(126)20-16-49)86(144)117-65(39-158)91(149)113-61(97(155)156)34-71(102)130/h2*13-20,42-47,52-67,75-77,123-126,157-160H,12,21-41,98H2,1-11H3,(H2,99,127)(H2,100,128)(H2,101,129)(H2,102,130)(H,103,148)(H,104,137)(H,105,143)(H,106,141)(H,107,152)(H,108,138)(H,109,146)(H,110,142)(H,111,145)(H,112,139)(H,113,149)(H,114,136)(H,115,150)(H,116,140)(H,117,144)(H,118,151)(H,119,153)(H,120,131)(H,121,147)(H,122,154)(H,132,133)(H,134,135)(H,155,156)/t2*46-,47-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-,65-,66-,67-,75-,76-,77-/m00/s1
InChI key
XYPANFGXFLOTDK-CJLNJPJHSA-N
Gene Information
bovine ... INS(280829)
cow ... INS(280829)
Application
Insulin solution from bovine pancreas has been used:
- to study the effect of exogenous insulin on plasma concentrations of glucose, insulin, insulin-like growth factor I (IGF-I), and progesterone (P4) in pubertal beef heifers[1]
- to study the insulin responsiveness of the placenta during pregnancy complications using primary human trophoblast cells and placental villous explants[2]
- to study the effect of insulin receptor signaling dendritic spine density in neonatal mouse hippocampal neurons in vitro[3]
Biochem/physiol Actions
Insulin is a major glucose-lowering hormone, insufficiency of which causes hyperglycemia observed in type 2 diabetes.[4]
Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.
Physical form
stable 2-8 °C
1 of 1
This Item | |||
|---|---|---|---|
| biological source bovine | biological source - | biological source bovine | biological source - |
| Quality Level 400 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line - | product line BioXtra | product line - |
| concentration 10 mg/mL insulin in 25 mM HEPES, pH 8.2 | concentration 100 μg/mL (PBS pH 7.2) | concentration - | concentration - |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) liquid chromatography (LC): suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| form solution | form liquid | form lyophilized powder | form powder |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
ppe
Eyeshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| I0516-5ML | 04061838101051 |
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Is there a specific solution that I should use to further dilute or is sterile saline ok?
1 answer-
Helpful?
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Can I freeze the aliquots at -20 or -80 for the long-term storage?
1 answer-
The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.
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What is the shelf life of this product if stored at 2-8°C ? Thanks.
1 answer-
This product is assigned an expiration date of 2 years from the date of manufacture. The expiration date is lot-specific and is printed on the label, as well as in the Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under ‘Certificate of Analysis’:
https://www.sigmaaldrich.com/product/sigma/i0516#product-documentationHelpful?
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Is bovine insulin (I0516) suitable for use in rat cell culture?
1 answer-
The product has not been validated for rat cell culture application. However, based on the following citations, it appears to work well with rat cell culture:
•Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes
published in J Vis Exp. 2020 Oct 10;(164):10.3791/61073. PMID: 33104067.•Collagen hydrogels with controllable combined cues of elasticity and topography to regulate cellular processes" published in Biomed Mater. 2021 Jun 11;16(4). PMID: 34030146.
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Does this solution contain zinc? How would that affect how quickly the insulin would act in a mammalian cell culture?
1 answer-
The product contains 10 mg/mL insulin in 25 mM HEPES, pH 8.2, but it does not include zinc. Currently, the effect of zinc on insulin signaling is not characterized. However, it is noted that zinc appears to be able to enhance insulin-mediated IR tyrosine phosphorylation according to the article Biol Trace Elem Res. 2004 Oct;101(1):19-36. Effect of chromium and zinc on insulin signaling in skeletal muscle cells. PMID: 15516700.
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is this product suitable for 3T3-L1 differentiation?
1 answer-
This product is supplied as a sterile bovine insulin solution at 10 mg/mL and would be suitable for diffentiation assays. This item has not been tested on 3T3-L1 cells, but is part of the differentiation protocol for the WT-1 preadipocyte cell line, product SCC255. See the link below to review this protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/568/334/scc255ds.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the comparison between International Units (IU) and USP units for insulin?
1 answer-
The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.
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What is the molarity of Product I0516, Insulin solution?
1 answer-
Product No. I0516 is a 10 mg/mL solution of bovine insulin (MW 5733.5). The molarity is 1.7 μmol/mL or 1.7 mM.
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How many units/mg am I getting in Product I0516, Insulin solution?
1 answer-
This solution is made using insulin Product No. I5500. The potency specification for the I5500 is not less than 27 USP units/mg. However, we do not test for potency of the final product of this solution. It is possible some activity is lost during the manufacturing process.
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